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Team:Brown/Protocols
From 2010.igem.org
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*[[:Team:Brown/Protocols/Ligation|Ligation]] | *[[:Team:Brown/Protocols/Ligation|Ligation]] | ||
Restriction digests | Restriction digests | ||
| - | *[[:Team:Brown/Protocols/Restriction_Digest|Restriction Digest]] | + | *[[:Team:Brown/Protocols/Restriction_Digest|Restriction [Double] Digest]] |
Overnights | Overnights | ||
*[[:Team:Brown/Protocols/Overnight_Culture|Overnight Culture]] | *[[:Team:Brown/Protocols/Overnight_Culture|Overnight Culture]] | ||
Revision as of 07:36, 25 October 2010
Protocols
Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our Notebook.
PCR
PCR Cleanup/Purification
Gel Extraction
Ligations
Restriction digests
Overnights
Transformations
Cell competence protocols
Minipreps
