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Team:Brown/Protocols
From 2010.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
PCR | PCR | ||
*[[:Team:Brown/Protocols/PCR|PCR]] | *[[:Team:Brown/Protocols/PCR|PCR]] | ||
| + | PCR Cleanup/Purification | ||
| + | *[[Media:QIAquick_Spin_Handbook.pdf|PCR Cleanup/Purification (page 19)]] | ||
| + | Gel Extraction | ||
| + | *[[Media:QIAquick_Spin_Handbook.pdf|Gel Extraction (page 25)]] | ||
Ligations | Ligations | ||
*[[:Team:Brown/Protocols/Ligation|Ligation]] | *[[:Team:Brown/Protocols/Ligation|Ligation]] | ||
| Line 18: | Line 22: | ||
*[[Media:Protocol_CaCl2_competent_cells.pdf|CaCl2 competence (bulk)]] | *[[Media:Protocol_CaCl2_competent_cells.pdf|CaCl2 competence (bulk)]] | ||
Minipreps | Minipreps | ||
| - | *[[Media:QIAprep_Miniprep_Handbook.pdf|Miniprep handbook ( | + | *[[Media:QIAprep_Miniprep_Handbook.pdf|Miniprep handbook (pages 22-23)]] |
Revision as of 07:35, 25 October 2010
Protocols
Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our Notebook.
PCR
PCR Cleanup/Purification
Gel Extraction
Ligations
Restriction digests
Overnights
Transformations
Cell competence protocols
Minipreps
