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Team:Brown/Protocols
From 2010.igem.org
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*[[Media:Protocol_Quick_CaCl2_competence.pdf|Quick CaCL2 Competence]] | *[[Media:Protocol_Quick_CaCl2_competence.pdf|Quick CaCL2 Competence]] | ||
*[[Media:Protocol_CaCl2_competent_cells.pdf|CaCl2 competence (bulk)]] | *[[Media:Protocol_CaCl2_competent_cells.pdf|CaCl2 competence (bulk)]] | ||
| + | Minipreps | ||
| + | *[[Media:QIAprep_Miniprep_Handbook.pdf|Miniprep handbook (pg22-23)]] | ||
Revision as of 07:23, 25 October 2010
Protocols
Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our Notebook.
PCR
Ligations
Restriction digests
Overnights
Transformations
Cell competence protocols
Minipreps
