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Team:Brown/Project/Light pattern
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Biological manufacturing of complex compounds often requires the synthesis of many intermediate products. Production of these intermediates is currently triggered by inefficient methods, such as chemical inputs (tetracycline, estrogen-analogs, arabinose, etc) or drastic changes to the cellular environment (pH, oxygen levels, temperature, etc). On an industrial scale, this chemical induction requires large quantities of reagents and extensive purification, while environmental induction requires conditions that can adversely affect cell vitality and yield. To this end, '''we are engineering an E. coli genetic circuit that can pass through four stable states of protein production triggered solely by ON/OFF patterns of light.''' With this production method, '''we can link multiple synthesis steps to a single, clean and rapidly scalable input.''' | Biological manufacturing of complex compounds often requires the synthesis of many intermediate products. Production of these intermediates is currently triggered by inefficient methods, such as chemical inputs (tetracycline, estrogen-analogs, arabinose, etc) or drastic changes to the cellular environment (pH, oxygen levels, temperature, etc). On an industrial scale, this chemical induction requires large quantities of reagents and extensive purification, while environmental induction requires conditions that can adversely affect cell vitality and yield. To this end, '''we are engineering an E. coli genetic circuit that can pass through four stable states of protein production triggered solely by ON/OFF patterns of light.''' With this production method, '''we can link multiple synthesis steps to a single, clean and rapidly scalable input.''' | ||
| - | === | + | ===Overview=== |
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| + | Our project this year attempts to tackle what we thought to be an emerging issue in synthetic biology. There is a growing trend among iGEM projects towards complex systems; the registry of standard parts is growing quickly and the tools at the disposal of a synthetic biologist are increasing rapidly. These fantastic and intricate projects that the iGEM and synthetic biologist community produces often require equally elaborate input; here, the user of a system | ||
===Workflow/Methods=== | ===Workflow/Methods=== | ||
Revision as of 07:01, 25 October 2010
Light Pattern Controlled Circuit
Contents |
Abstract
Biological manufacturing of complex compounds often requires the synthesis of many intermediate products. Production of these intermediates is currently triggered by inefficient methods, such as chemical inputs (tetracycline, estrogen-analogs, arabinose, etc) or drastic changes to the cellular environment (pH, oxygen levels, temperature, etc). On an industrial scale, this chemical induction requires large quantities of reagents and extensive purification, while environmental induction requires conditions that can adversely affect cell vitality and yield. To this end, we are engineering an E. coli genetic circuit that can pass through four stable states of protein production triggered solely by ON/OFF patterns of light. With this production method, we can link multiple synthesis steps to a single, clean and rapidly scalable input.
Overview
Our project this year attempts to tackle what we thought to be an emerging issue in synthetic biology. There is a growing trend among iGEM projects towards complex systems; the registry of standard parts is growing quickly and the tools at the disposal of a synthetic biologist are increasing rapidly. These fantastic and intricate projects that the iGEM and synthetic biologist community produces often require equally elaborate input; here, the user of a system
Workflow/Methods
Modeling
See our modeling page at: blah blah
Results
