Team:Brown/Protocols

From 2010.igem.org

(Difference between revisions)
(Protocols)
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Cell competence protocols
Cell competence protocols
*[[Media:Protocol_Quick_CaCl2_competence.pdf|Quick CaCL2 Competence]]
*[[Media:Protocol_Quick_CaCl2_competence.pdf|Quick CaCL2 Competence]]
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*[[Media:Protocol_CaCl2_competent_cells.pdf|CaCl2 competence (bulk)]]

Revision as of 04:47, 25 October 2010

Protocols

Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our Notebook. PCR

Ligations

Restriction digests

Overnights

Transformations

Cell competence protocols