"
Team:MIT phage construction
From 2010.igem.org
(Difference between revisions)
| Line 63: | Line 63: | ||
<br> | <br> | ||
<br><b><a href="http://partsregistry.org/Part:BBa_K415100">BBa_K415100</a></b> | <br><b><a href="http://partsregistry.org/Part:BBa_K415100">BBa_K415100</a></b> | ||
| - | <br>Components: RBS : M13 PVIII | + | <ul><li> |
| - | <br>Explanation: This is a basic translational unit: a ribosome-binding site and the p8 gene. You can attach any promoter in front of it for expression. | + | <br>Components: RBS : M13 PVIII</li> |
| + | <li> | ||
| + | <br>Explanation: This is a basic translational unit: a ribosome-binding site and the p8 gene. You can attach any promoter in front of it for expression.</li></ul> | ||
<br><img src="http://partsregistry.org/wiki/images/7/7c/K415100.png"> | <br><img src="http://partsregistry.org/wiki/images/7/7c/K415100.png"> | ||
| + | |||
| + | |||
<br><br><b><a href="http://partsregistry.org/Part:BBa_K415108">BBa_K415108</a></b> | <br><br><b><a href="http://partsregistry.org/Part:BBa_K415108">BBa_K415108</a></b> | ||
| - | <br>Components: RBS : M13 PIII | + | <ol><li> |
| - | <br>Explanation: This is a basic translational unit: a ribosome-binding site and the p3 gene. You can attach any promoter in front of it for expression. | + | <br>Components: RBS : M13 PIII</li> |
| + | <li> | ||
| + | <br>Explanation: This is a basic translational unit: a ribosome-binding site and the p3 gene. You can attach any promoter in front of it for expression.</li></ol> | ||
<br><img src="http://partsregistry.org/wiki/images/7/7c/K415100.png"> | <br><img src="http://partsregistry.org/wiki/images/7/7c/K415100.png"> | ||
| + | |||
| + | |||
<br><br><b><a href="http://partsregistry.org/Part:BBa_K415138">BBa_K415138</a></b> | <br><br><b><a href="http://partsregistry.org/Part:BBa_K415138">BBa_K415138</a></b> | ||
| - | <br>Components: pLac : RBS : M13 PIII | + | <ol><li> |
| - | <br>Explanation: This is a basic expression unit: a promoter in front of a ribosome-binding site and the p3 gene. We used this to generate constitutive expression of p3 in cells infected with hyperphage in an attempt to rescue the wild-type, non-hairy phenotype. See the results page for an AFM image of this working. | + | <br>Components: pLac : RBS : M13 PIII</li> |
| + | <li> | ||
| + | <br>Explanation: This is a basic expression unit: a promoter in front of a ribosome-binding site and the p3 gene. We used this to generate constitutive expression of p3 in cells infected with hyperphage in an attempt to rescue the wild-type, non-hairy phenotype. See the results page for an AFM image of this working.</li></ul> | ||
| + | |||
| + | |||
<br><img src="https://static.igem.org/mediawiki/2010/4/4a/138.png"> | <br><img src="https://static.igem.org/mediawiki/2010/4/4a/138.png"> | ||
<br><br><b>BBa_K415147</b> | <br><br><b>BBa_K415147</b> | ||
| Line 78: | Line 90: | ||
<br>Explanation: | <br>Explanation: | ||
<br><img src=""> | <br><img src=""> | ||
| + | |||
| + | |||
<br><br><b>BBa_K415148</b> | <br><br><b>BBa_K415148</b> | ||
<br>Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-Jun | <br>Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-Jun | ||
<br>Explanation: | <br>Explanation: | ||
<br><img src=""> | <br><img src=""> | ||
| + | |||
| + | |||
<br><br><b>BBa_K415149</b> | <br><br><b>BBa_K415149</b> | ||
<br>Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-ACID | <br>Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-ACID | ||
<br>Explanation: | <br>Explanation: | ||
<br><img src=""> | <br><img src=""> | ||
| + | |||
| + | |||
<br><br><b>BBa_K415150</b> | <br><br><b>BBa_K415150</b> | ||
<br>Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-BASE | <br>Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-BASE | ||
<br>Explanation: | <br>Explanation: | ||
<br><img src=""> | <br><img src=""> | ||
| + | |||
| + | |||
<br><br><b>BBa_K415151</b> | <br><br><b>BBa_K415151</b> | ||
<br>Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-GR1 | <br>Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-GR1 | ||
<br>Explanation: | <br>Explanation: | ||
<br><img src=""> | <br><img src=""> | ||
| + | |||
| + | |||
<br><br><b>BBa_K415152</b> | <br><br><b>BBa_K415152</b> | ||
<br>Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII-GR2 | <br>Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII-GR2 | ||
Revision as of 23:21, 24 October 2010
iGEM 2010
hairy cells and polymerizing phage - construction |
|
PARTS BBa_K415100
BBa_K415108
BBa_K415138
BBa_K415147 Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-Fos Explanation: BBa_K415148 Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-Jun Explanation: BBa_K415149 Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-ACID Explanation: BBa_K415150 Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-BASE Explanation: BBa_K415151 Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR: Term : Plux/cI : RBS : M13 PVIII-GR1 Explanation: BBa_K415152 Components: PluxR/cI : RBS : mCherry : Term : PtetR : RBS : LuxR:Term : Plux/cI : RBS : M13 PVIII-GR2 Explanation: |
