Team:Caltech/Protocols

From 2010.igem.org

(Difference between revisions)
 
(5 intermediate revisions not shown)
Line 2: Line 2:
Content=
Content=
__NOTOC__
__NOTOC__
-
* [[Team:Caltech/Competent Cells]]
+
* [[Team:Caltech/Competent Cells|Competent Cells]]
* [[Team:Caltech/Electroporation|Transformation by Electroporation]]
* [[Team:Caltech/Electroporation|Transformation by Electroporation]]
-
* Making stock solutions
+
* [[Team:Caltech/Glycerol Stocks|Freezer (glycerol) stocks]]
-
* Freezer (glycerol) stocks
+
* [[Team:Caltech/PlatingAntibiotics|Plating antibiotics]]
-
* Plating antibiotics
+
* [[Team:Caltech/SeqPrimers|Sequencing primers]]
-
* Sequencing primers
+
<!--* [[Team:Caltech/MakingPlastic|Growing cells with PHA (and substrate oil)]]-->
-
* Making synthase casette
+
* [[Team:Caltech/Colony PCR|Colony PCR]]
-
** Sources of sequences
+
* [[Team:Caltech/Cloning|Two-day Cloning]]
-
** Optimizing & checking genes
+
-
* Chemical/Crosslinking reactions
+
-
** Digesting substrate (hydrolysis) + epoxidation (MCPBA)
+
-
** crosslinking (AIBN3) + UV initiation
+
-
* Inducing lysis via 632nm light initiation
+
-
* Making cell/glycerol/agar mixture
+
-
** Applying this to plates
+
-
* Growing cells with PHA (and substrate oil)
+
-
* [[Team:Caltech/Colony PCR]]
+
}}
}}

Latest revision as of 23:20, 24 October 2010


iGEM 2010



Home

People

Project Details

Protocols

Completed Systems

Notebook

Biosafety

Human Impact

References

Support


Caltech logo watermark.png
Error creating thumbnail: /websites/2010.igem.org/wiki/bin/ulimit4.sh: line 4: convert: command not found
Caltech footer.png


Locations of visitors to this page