Team:Groningen/27 September 2010

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'''Laura'''
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Debugging the matlab model of killswitch and gene expression.
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Revision as of 20:29, 24 October 2010

iGEM Groningen 2010

Hydrophobofilm
pushing coatings into a greener future

Week 29

Chaplin ladder on gel-Peter


The Yqxm and the Srfa (both variants)promoters where extracted from genomic Bacillus Subtilis DNA via PCR and inserted into the PNZ8901 plasmid. All transformations where done in Lactococcus Lactis. Restriction analysis was performed to check the constructs. The concentrations where determined with the Nanodrop


Srfa 1 Srfa 2 Yqxm
Clone 1 75.3mg/ml 78.9mg/ml 72.0mg/mL
Clone 2 25.6mg/ml 40.2mg/ml 57.6mg/mL
Clone 3 44.4mg/ml False positive False positive


Laura

Debugging the matlab model of killswitch and gene expression.

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