Team:Groningen/27 September 2010
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Revision as of 20:29, 24 October 2010
Week 29
Chaplin ladder on gel-Peter
The Yqxm and the Srfa (both variants)promoters where extracted from genomic Bacillus Subtilis DNA via PCR and inserted into the PNZ8901 plasmid. All transformations where done in Lactococcus Lactis.
Restriction analysis was performed to check the constructs. The concentrations where determined with the Nanodrop
Srfa 1 | Srfa 2 | Yqxm | |
Clone 1 | 75.3mg/ml | 78.9mg/ml | 72.0mg/mL |
Clone 2 | 25.6mg/ml | 40.2mg/ml | 57.6mg/mL |
Clone 3 | 44.4mg/ml | False positive | False positive |
Laura
Debugging the matlab model of killswitch and gene expression.