(Difference between revisions)

Latest revision as of 19:48, 24 October 2010

Week 10

Liquid cultures for a new western started (planned for tomorrow, with new antibodies and another machine). Preparing a positive control for the western blot (bacteria expressing a his-tag-protein).

Western Blot started. Samples: Immunotoxin, p25, p28, positive and negative control. Did the blot at the Lemaitre lab with Onya. Blot done twice: once with the Maerkl lab antibodies and once with Onya's antibodies.

Western Blot finished. The blot with Onya's antibodies did not show any signal except a very weak band in the positive control. --> Check the size!!

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 == Cloning ==
-*Western Blot with Immunotoxin, p25, 028, positive and negative control. Did the blot at the Lemaitre lab with Onya. Blot done twice once with the Maerkl lab antibodies and once with Onya's antibodies.
+*Western Blot started. Samples: Immunotoxin, p25, p28, positive and negative control. Did the blot at the Lemaitre lab with Onya. Blot done twice: once with the Maerkl lab antibodies and once with Onya's antibodies.
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 == Cloning ==
+*Western Blot finished. The blot with Onya's antibodies did not show any signal except a very weak band in the positive control. --> Check the size!!
 | style="width: 50%" valign="top" |
 == Other ==
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+{{EPFL_2010_bottom_wrap}}
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