(Difference between revisions)

Latest revision as of 19:48, 24 October 2010

Week 10

Liquid cultures for a new western started (planned for tomorrow, with new antibodies and another machine). Preparing a positive control for the western blot (bacteria expressing a his-tag-protein).

Western Blot started. Samples: Immunotoxin, p25, p28, positive and negative control. Did the blot at the Lemaitre lab with Onya. Blot done twice: once with the Maerkl lab antibodies and once with Onya's antibodies.

Western Blot finished. The blot with Onya's antibodies did not show any signal except a very weak band in the positive control. --> Check the size!!

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 == Cloning ==
+*Western Blot started. Samples: Immunotoxin, p25, p28, positive and negative control. Did the blot at the Lemaitre lab with Onya. Blot done twice: once with the Maerkl lab antibodies and once with Onya's antibodies.
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 == Other ==
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 == Cloning ==
+*Western Blot finished. The blot with Onya's antibodies did not show any signal except a very weak band in the positive control. --> Check the size!!
 | style="width: 50%" valign="top" |
 == Other ==
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+{{EPFL_2010_bottom_wrap}}
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