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Team:Heidelberg/Notebook/miMeasure
From 2010.igem.org
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(→Seeding and transfection of cells for TECAN, flow cytometry, plate reader and microscopy) |
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5000 cells were seeded on day one in each well of the 96 well plate. Transfection of the constructs (M12-M22) with four different conditions were carried out on day two. The ratio of transfection is 1 (M construct) : 5 (stuffer/ miRsAg/ pcDNA5/ shRNA3) with a total amount | 5000 cells were seeded on day one in each well of the 96 well plate. Transfection of the constructs (M12-M22) with four different conditions were carried out on day two. The ratio of transfection is 1 (M construct) : 5 (stuffer/ miRsAg/ pcDNA5/ shRNA3) with a total amount | ||
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Condition a: cotransfected with stuffer (salmon sperm DNA) | Condition a: cotransfected with stuffer (salmon sperm DNA) | ||
Condition b: costransfected with synthetic RNA miRsAg | Condition b: costransfected with synthetic RNA miRsAg | ||
Condition c: cotransfected with empty pcDNA5 | Condition c: cotransfected with empty pcDNA5 | ||
Condition d: cotransfected with synthetic shRNA3 | Condition d: cotransfected with synthetic shRNA3 | ||
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A control consisting of the empty miMeasure plasmid (without binding site) was also cotransfected wit the same conditions a, b, c and d. | A control consisting of the empty miMeasure plasmid (without binding site) was also cotransfected wit the same conditions a, b, c and d. | ||
Revision as of 18:58, 24 October 2010
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