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Team:MIT phage design
From 2010.igem.org
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| + | The above shows the RBS + p8-fusion genetic design. This genetic fusion is designed to allow the expression of a leucine zipper (coil in the diagram) on the p8 phage coat protein. There are six total p8 fusions for the six different zippers. To assist in proper cellular trafficking to the membrane, a leader sequence was introduced. Additionally, HA and Myc tags were introduced (one for each part of a zipper pair), which were used in western blotting experiments to test for the expression of the p8-fusion genes. | ||
Revision as of 17:08, 24 October 2010
iGEM 2010
hairy cells and polymerizing phage |
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DESIGN 
The above shows the RBS + p8-fusion genetic design. This genetic fusion is designed to allow the expression of a leucine zipper (coil in the diagram) on the p8 phage coat protein. There are six total p8 fusions for the six different zippers. To assist in proper cellular trafficking to the membrane, a leader sequence was introduced. Additionally, HA and Myc tags were introduced (one for each part of a zipper pair), which were used in western blotting experiments to test for the expression of the p8-fusion genes. |
