Team:SDU-Denmark/project-i

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(The Idea)
(Hyperflagellation)
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== Hyperflagellation ==
== Hyperflagellation ==
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To achieve hyperflagellation we have decided to focus mainly on increasing the expression of the ''flhD'' and ''flhC'' transcriptional regulators, also known as the master regulon of flagella synthesis(?). In normal ''E. coli'' the ''flhDC'' operon is tightly regulated by numerous factors(), resulting in average expression of 3-7 flagella(). In some hyperflagellated strains, mutations have been found upstream of the regulon that increase expression(), making the cells hypermotile. We have decided to take a down-and-dirty approach to increase flagella expression, overriding the regulation alltogether by putting the two genes on a constitutive promotor. We hereby hope to increase the pumping power of our system.
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To achieve hyperflagellation we have decided to focus mainly on increasing the expression of the ''flhD'' and ''flhC'' transcriptional regulators, also known as the master regulon of flagella synthesis[https://2010.igem.org/Team:SDU-Denmark/project-i#References (7)]. In normal ''E. coli'' the ''flhDC'' operon is tightly regulated by numerous factors[https://2010.igem.org/Team:SDU-Denmark/project-i#References (7)], resulting in average expression of 4 flagellae per cell[https://2010.igem.org/Team:SDU-Denmark/project-i#References (8)]. In some hyperflagellated strains, mutations have been found upstream of the regulon that increase expression(), making the cells hypermotile. We have decided to take a down-and-dirty approach to increase flagella expression, overriding the regulation alltogether by putting the two genes on a constitutive promotor. We hereby hope to increase the pumping power of our system.
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== Phototaxis ==
== Phototaxis ==
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Revision as of 12:27, 24 October 2010