Team:Heidelberg/Notebook/Capsid Shuffling/Homology Based

From 2010.igem.org

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= Homology Based Capsid Shuffling =
= Homology Based Capsid Shuffling =
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==31/08/2010==
 
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*we decided to use AAV serotypes 1,2,5,6,8 and 9 as those have been described in the literature to infect pancreas cells. <br />
 
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*cap plasmids (AAV 1, 2, 5, 6, 8, 9) have been diluted to a concentration of 200ng/ul
 
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*PCR on cap genes was performed using M13 primers as the genes are in the pBluescript vector:
 
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**AAV1 A
 
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**AAV2 A & B
 
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**AAV5 A & B
 
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**AAV6
 
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**AAV8 A & B
 
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**AAV9 A
 
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Revision as of 12:18, 24 October 2010

Homology Based Capsid Shuffling

August
MTWTFSS
1
2345678
9101112131415
16171819202122
23242526272829
3031
September
MTWTFSS
12345
6789101112
13141516171819
20212223242526
27282930
-
October
MTWTFSS
010203
04050607080910
11121314151617
18192021222324
25262728293031
-


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