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Team:UPO-Sevilla/Notebook/09 01
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<p>We wrote protocols for next devices, SDM of UPO28 and digestion analysis of 1+19 and 16+3.</p> | <p>We wrote protocols for next devices, SDM of UPO28 and digestion analysis of 1+19 and 16+3.</p> | ||
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<a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a> | <a class="return_button" href="/Team:UPO-Sevilla/Notebook" title="Notebook"><span>Return to Notebook</span></a> | ||
Revision as of 12:17, 24 October 2010
September, 01st
Production Team
David Caballero. We wrote the protocol for fecI-fecR* (UPO28*) SDM. It was necessary to remove a PstI site in 1184pb (inside of fecR* secuence). We were going to performance two strategies:
- fecI-fecR* SDM to get a composed part.
- fecR* SDM to get only a fecR part. We already had fecI part.
It was not necessary to mutagenize fecA* (UPO8*) or gltD** (UPO17) since we were not going to keep on building devices of them.
Assembly Team
We analyzed all the information we had about parts and devices and toke a decision: we were going to do without device 2 (Fec system) and devices 10, 11 and 12 (Glutamate Synthase). The explication was:
- D2 was a control of the sensing system. Moreover we could not synthesize fecA part.
- D10, D11 and D12: glutamate synthase subunits were pretty big so they could give us problems. Also we had problems to remove EcoRI target in one of them by SDM. It was not a huge deal not to continue with that chemoattractant synthesize system because we had other two: aspartate to E. coli and salicilate to P. putida.
We wrote protocols for next devices, SDM of UPO28 and digestion analysis of 1+19 and 16+3.
Go to 13th of August Go to 2nd of September Return to Notebook
Sponsors
