"
Project/Venus/
From 2010.igem.org
(Difference between revisions)
(→Sequence and Annotation) |
|||
| Line 6: | Line 6: | ||
=== BioBrick Background === | === BioBrick Background === | ||
<br /> | <br /> | ||
| - | === Sequence and Annotation === | + | === Sequence Development and Annotation === |
| + | As was the case in the assembly of the PlcR-PapR BioBrick, the synthesized Venus BioBrick was received in the pMK plasmid (below). | ||
| + | [[Image:PMK-Venus.JPG]] <br /> <br /> | ||
| + | |||
| + | Once digested with EcoR1 and Pst1, the Venus fragment was recovered via gel extraction and purified in preparation for its ligation into PSB1C3. Lane 2 houses the Venus digest with the excised fragment shown (782bp). <br /> | ||
| + | [[Image:Venus_digest.JPG]] <br /> <br /> | ||
| + | |||
<br /> | <br /> | ||
| + | |||
=== Testing and verification === | === Testing and verification === | ||
<br /> | <br /> | ||
Revision as of 10:32, 24 October 2010
Contents |
Characterisation of the fluorescent reporter protein - Venus - [http://partsregistry.org/Part:BBa_K354002 BBa_K354002]
BioBrick Background
Sequence Development and Annotation
As was the case in the assembly of the PlcR-PapR BioBrick, the synthesized Venus BioBrick was received in the pMK plasmid (below).
Once digested with EcoR1 and Pst1, the Venus fragment was recovered via gel extraction and purified in preparation for its ligation into PSB1C3. Lane 2 houses the Venus digest with the excised fragment shown (782bp).
Testing and verification
