Team:Brown/Notebook/September21

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(Redo of Transformation of J13002-AraC and J13002-LacI ligations)
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==Tuesday, September 21, 2010==
==Tuesday, September 21, 2010==
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===Miniprep of AraC-J13002 DNA===
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3 tubes of 30 μl DNA, placed in WRD box at 2:10 PM.
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===Redo of Transformation of J13002-AraC and J13002-LacI ligations===
===Redo of Transformation of J13002-AraC and J13002-LacI ligations===
Retransformation of XL1 Blues with J13002-LacI ligation. Used remaining DNA.
Retransformation of XL1 Blues with J13002-LacI ligation. Used remaining DNA.

Latest revision as of 23:12, 23 October 2010

Tuesday, September 21, 2010

Miniprep of AraC-J13002 DNA

3 tubes of 30 μl DNA, placed in WRD box at 2:10 PM.

Redo of Transformation of J13002-AraC and J13002-LacI ligations

Retransformation of XL1 Blues with J13002-LacI ligation. Used remaining DNA.