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Team:St Andrews/project/laboratory/protocols
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| + | ==Protocols== | ||
| + | |||
| + | We used a number of protocols from OpenWetWare in addition to the manufacturer's recommended protocols for our materials. | ||
| + | |||
| + | ===Cell Culture=== | ||
| + | OpenWetWare [http://openwetware.org/wiki/Bacterial_cell_culture Bacterial Cell Culture] | ||
| + | ===Transformation=== | ||
| + | |||
| + | [http://www.neb.com/nebecomm/products/protocolProductC2984.asp NEB Turbo Competent E. coli] | ||
| + | |||
| + | [http://www.clontech.com/products/detail.asp?product_id=215795&tabno=3 Clontech Stellar Competent E. coli.] | ||
| + | |||
| + | ===Restriction Digest=== | ||
| + | |||
| + | Promega Enzymes | ||
| + | |||
| + | NEB Biobrick Assembly Kit (Gingko Bioworks) | ||
| + | |||
| + | ===Ligation=== | ||
| + | |||
| + | NEB Biobrick Assembly Kit (Gingko Bioworks) | ||
| + | |||
| + | ===PCR=== | ||
| + | |||
| + | OpenWetWare [http://openwetware.org/wiki/Knight:Colony_PCR Knight - Colony PCR] | ||
| + | |||
| + | NEB Taq | ||
| + | |||
| + | Finnzymes Phusion | ||
| + | |||
| + | ===DNA Extraction=== | ||
| + | [http://www.qiagen.com/products/plasmid/qiaprepminiprepsystem/qiaprepspinminiprepkit.aspx#Tabs=t2 Quiagen Miniprep] | ||
| + | |||
| + | Isopropanol Extraction of small DNA fragments | ||
| + | |||
| + | ===Construction of BioBricks=== | ||
| + | |||
| + | |||
| + | Gingko Bioworks - [http://ginkgobioworks.com/support/ 3A Biobrick Assembly] (NEB Biobrick Assembly Kit) | ||
| + | |||
| + | OpenWetWare [http://openwetware.org/wiki/In-fusion_biobrick_assembly In Fusion Biobrick Assembly] (Primer design and assembly strategy) | ||
| + | |||
| + | Clontech [http://www.clontech.com/products/detail.asp?catalog_id=639621 In Fusion Cloning] (In Fusion reaction itself) | ||
| + | |||
| + | OpenWetWare [http://openwetware.org/wiki/Endy:Annealing_complementary_primers Endy - Annealing Complementary Primers] | ||
Latest revision as of 20:12, 23 October 2010
Contents |
Protocols
We used a number of protocols from OpenWetWare in addition to the manufacturer's recommended protocols for our materials.
Cell Culture
OpenWetWare [http://openwetware.org/wiki/Bacterial_cell_culture Bacterial Cell Culture]
Transformation
[http://www.neb.com/nebecomm/products/protocolProductC2984.asp NEB Turbo Competent E. coli]
[http://www.clontech.com/products/detail.asp?product_id=215795&tabno=3 Clontech Stellar Competent E. coli.]
Restriction Digest
Promega Enzymes
NEB Biobrick Assembly Kit (Gingko Bioworks)
Ligation
NEB Biobrick Assembly Kit (Gingko Bioworks)
PCR
OpenWetWare [http://openwetware.org/wiki/Knight:Colony_PCR Knight - Colony PCR]
NEB Taq
Finnzymes Phusion
DNA Extraction
[http://www.qiagen.com/products/plasmid/qiaprepminiprepsystem/qiaprepspinminiprepkit.aspx#Tabs=t2 Quiagen Miniprep]
Isopropanol Extraction of small DNA fragments
Construction of BioBricks
Gingko Bioworks - [http://ginkgobioworks.com/support/ 3A Biobrick Assembly] (NEB Biobrick Assembly Kit)
OpenWetWare [http://openwetware.org/wiki/In-fusion_biobrick_assembly In Fusion Biobrick Assembly] (Primer design and assembly strategy)
Clontech [http://www.clontech.com/products/detail.asp?catalog_id=639621 In Fusion Cloning] (In Fusion reaction itself)
OpenWetWare [http://openwetware.org/wiki/Endy:Annealing_complementary_primers Endy - Annealing Complementary Primers]
