From 2010.igem.org
(Difference between revisions)
|
|
| Line 50: |
Line 50: |
| | || Part || ODstart | | || Part || ODstart |
| | |- | | |- |
| - | || <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> || | + | || <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> || 0.1456 |
| | |- | | |- |
| - | || <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> || | + | || <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> || 0 |
| | |- | | |- |
| - | || <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> || | + | || <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> || 0.3257 |
| | |- | | |- |
| - | || <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> || | + | || <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> || - |
| | |- | | |- |
| - | || <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> || | + | || <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> || 0.5068 |
| | |- | | |- |
| - | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> || | + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> || 0.1609 |
| | |- | | |- |
| - | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> || | + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> || 0.3098 |
| | |- | | |- |
| - | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> || | + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> || 0.1463 |
| | |- | | |- |
| - | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> || | + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> || - |
| | |- | | |- |
| - | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> || | + | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> || - |
| - | |- | + | |
| - | || <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> ||
| + | |
| | |} | | |} |
| | | | |
Revision as of 16:28, 23 October 2010
|
|
|
|
|
AUGUST, 17TH
PLATE
EXPERIMENT DESCRIPTION
Motivation
The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.
Methods
The experiment was performed in LB according to Protocol 1 on the following measurement parts:
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12)
- <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
- <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19-4C5) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
- <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)
RESULTS
Data were analyzed following the data analysis procedure to evaluate the ODstart. The plots correpsoning to the parts tested are reported:
The ODstart are reported in the table below:
| Part | ODstart
|
| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> | 0.1456
|
| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> | 0
|
| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> | 0.3257
|
| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> | -
|
| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> | 0.5068
|
| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> | 0.1609
|
| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> | 0.3098
|
| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> | 0.1463
|
| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> | -
|
| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> | -
|
|
|
|
"
