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Team:Groningen/27 September 2010
From 2010.igem.org
(Difference between revisions)
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The Yqxm and the Srfa (both variants)promoters where extracted from genomic ''Bacillus Subtilis'' DNA via PCR and inserted into the PNZ8901 plasmid. All transformations where done in ''Lactococcus Lactis''. | The Yqxm and the Srfa (both variants)promoters where extracted from genomic ''Bacillus Subtilis'' DNA via PCR and inserted into the PNZ8901 plasmid. All transformations where done in ''Lactococcus Lactis''. | ||
| + | Restriction analysis was performed to check the constructs. The concentrations where determined with the Nanodrop | ||
| + | {| class="wikitable" | ||
| + | |- | ||
| + | ! Srfa 1 | ||
| + | ! Srfa 2 | ||
| + | ! Yqxm | ||
| + | |- | ||
| + | | Clone 1 | ||
| + | | 75.3mg/ml | ||
| + | | 78.9mg/ml | ||
| + | | 72.0mg/mL | ||
| + | |- | ||
| + | | Clone 2 | ||
| + | | 25.6mg/ml | ||
| + | | 40.2mg/ml | ||
| + | | 57.6mg/mL | ||
| + | |- | ||
| + | | Clone 3 | ||
| + | | 44.4mg/ml | ||
| + | | False positive | ||
| + | | False positive | ||
| + | |} | ||
| + | |||
{{Team:Groningen/Footer}} | {{Team:Groningen/Footer}} | ||
Revision as of 10:47, 23 October 2010
Hydrophobofilm
pushing coatings into a greener future
Week 29
Chaplin ladder on gel-Peter
The Yqxm and the Srfa (both variants)promoters where extracted from genomic Bacillus Subtilis DNA via PCR and inserted into the PNZ8901 plasmid. All transformations where done in Lactococcus Lactis. Restriction analysis was performed to check the constructs. The concentrations where determined with the Nanodrop
| Srfa 1 | Srfa 2 | Yqxm | |
|---|---|---|---|
| Clone 1 | 75.3mg/ml | 78.9mg/ml | 72.0mg/mL |
| Clone 2 | 25.6mg/ml | 40.2mg/ml | 57.6mg/mL |
| Clone 3 | 44.4mg/ml | False positive | False positive |
