Team:Tsinghua

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=Tsinghua University=
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==Available Languages==
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[[Image:Usa.gif|middle|30px]] [[Team:Tsinghua#English|English]] 
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[[Image:China.gif|middle|30px]] [[Team:Tsinghua#我们的项目|中文]]
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<h1><IMG SRC="https://static.igem.org/mediawiki/2010/d/d8/TSSLogo.png" width="40px">Team</h1>
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[[Image:Russia.gif|middle|30px]] [[Team:Tsinghua#О проекте|Русский]]
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[[Image:France.gif|middle|30px]] [[Team:Tsinghua#Notre Projet|Français]]
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<div id="leftpic"><img src="https://static.igem.org/mediawiki/2010/4/49/Teampic.jpg" width=90px /></div>
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[[Image:Germany.gif|middle|30px]] [[Team:Tsinghua#Unser Projekt|Deutsch]]
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<p>We're from Tsinghua University.</p><p>Since China opened up to the world in 1978, Tsinghua University has developed at a breathtaking pace into a comprehensive research university. At present, the university has 14 schools and 56 departments with faculties in science, engineering, humanities, law, medicine, history, philosophy, economics, management, education and art. The University has now over 25,900 students, including 13,100 undergraduates and 12,800 graduate students. As one of China’s most renowned universities, Tsinghua has become an important institution for fostering talent and scientific research.</p><p>
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[[Image:Spain.gif|middle|30px]] [[Team:Tsinghua#Nuestro Proyecto|Español]]
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With the motto of “Self-Discipline and Social Commitment” and the spirit of “Actions Speak Louder than Words”, Tsinghua University is dedicated to the well-being of Chinese society and to world development.</p>
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[[Image:Japan.gif|middle|30px]] [[Team:Tsinghua#私たちのプロジェクト|日本語]]
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<p><span style="float:right"><a href="/Team:Tsinghua/team" >More...</a></span></p>
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<div class="rightblock">
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<a name="proj"></a>
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<h1><IMG SRC="https://static.igem.org/mediawiki/2010/d/d8/TSSLogo.png" width="40px" />Project</h1>
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<div id="leftpic"><img src="https://static.igem.org/mediawiki/2010/5/5e/TSModule2m.PNG" width=90px/></div><p>
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Monoclonal antibodies (mAb or moAb) are monospecific antibodies that are the same, made by identical immune cells that are all clones of a unique parent cell. When stimulated by almost any type of antigen, the immune system can create the specific antibody. This lays the foundation for monoclonal artificial antibodies.</p><p>
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The current most well-developed technique in Artificial Monoclonal Antibody is the famous Hybridoma Cell Production. Monoclonal antibodies are typically made by fusing myeloma cells with the spleen cells from a mouse that has been immunized with the desired antigen. The success rate is so low that a selective medium in which only fused cells can grow is used. This mixture of cells is then diluted and clones are grown from single parent cells on microtitre wells. The antibodies secreted by the different clones are then assayed for their ability to bind to the antigen. The most productive and stable clone is then selected for future use.</p>
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<p><span style="float:right"><a href="/Team:Tsinghua/project" >More...</a></span></p>
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<h1><IMG SRC="https://static.igem.org/mediawiki/2010/d/d8/TSSLogo.png" width=40px">Parts</h1>
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<div id="leftpic"><img src="http://biobricks.org/img/750px-BBFoundation_shadow.png" width=90px /></div><p>We built a host of parts during our project. The idea is that after every small step, we store our sequence as a biobrick part.</p><p> This strategy marks our progress and facilitates future use of these sequences.</p>
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<p><span style="float:right"><a href="/Team:Tsinghua/parts" >More...</a></span></p>
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=='''Our Project'''==
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[[Image:Usa.gif|15px]]English  [[Team:Tsinghua#Tsinghua University|Back]]
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<a name="exp"></a>
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<h1><IMG SRC="https://static.igem.org/mediawiki/2010/d/d8/TSSLogo.png" width="40px" />Experiment</h1>
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<div class="content_block">
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<div id="leftpic"><img src="http://www.htys.org/images/logo1.jpg" width=90px /></div><p>
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Our experiments are carefully recorded on a daily basis. Through the series of records, we can see our joys and sorrows.</p><p>Besides, we made the records for the purpose that our experiments can be repeated one day by someone else, thus contributing to the exploration of the unknown.</p>
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<p><span style="float:right"><a href="/Team:Tsinghua/experiments" >More...</a></span></p>
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</div></div>
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Tsinghua iGEM 2010 Project is focused on developing a new Antibody Production Method using Synthetic Biology.
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The Aim is to
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''' Simulate Antibody Selection and Production Technology with Bacteria'''.
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<a name="supp"></a>
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<h1><IMG SRC="https://static.igem.org/mediawiki/2010/d/d8/TSSLogo.png" width="40px">Support</h1>
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<div class="content_block">
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<div id="leftpic"><img src="https://static.igem.org/mediawiki/2010/9/9c/Humanpic.JPG" width=90px /></div><p>Our project is supported by School of Life Sciences, Department of Physics, Academy of Arts and Designs in Tsinghua.</p><P>
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Besides, we cited from a series of references.</p>
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<p><span style="float:right"><a href="/Team:Tsinghua/support" >More...</a></span></p>
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</div></div>
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Traditional method of antibody production such as Hybridoma Technology is expensive and time consuming. In Hybridoma Technology, generation of B-Cell library is accomplished inside experimental animal, mouse for example; using Flow Cytometry to select a specific kind of antibody; then hybridoma would be prepared to harvest antibody.
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<div class="rightblock">
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<a name="hum"></a>
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<h1><IMG SRC="https://static.igem.org/mediawiki/2010/d/d8/TSSLogo.png" width="40px">Human Practice</h1>
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<div class="content_block">
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<div id="leftpic"><img src="https://static.igem.org/mediawiki/2010/f/fd/Humanity.jpg" width=90px /></div><p>We put safety as our first priority and made a detailed safety brochure. </p>
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<p>We also devoted efforts to publicize synthetic biology and to cooperate with other teams. The teams in China held a summer meetup to discuss our progress and share our resources. We also held a lecture introducing iGEM and our project in Tsinghua Univeristy. To get further support, we sought the cooperation of iGEM Team at Macquire, Australia.</p>
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<p><span style="float:right"><a href="/Team:Tsinghua/HumanPractice" >More...</a></span></p>
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In this project, we expect to achieve all these processes cheap and efficiently in E.coli Cell. The whole project can be divided into two modules:
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====MODULE I: Generation of Antibody Library====
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We pre-modified the genome of E. coli by inserting a sequence with the I-Sel restriction sites. Then, into the plasmid of E. coli we inserted in both ends with two fragments which contain the recombinant sites (antibody variable region V, D, J). After being induced, recombination can be achieved by the crossed sequence. Specifically, as to we experiment, we will use fluorescent protein and resistance genes to testify.
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====MODULE II: Selection of Specific Antibody====
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<html>See Details @ <a href="https://2010.igem.org/Team:Tsinghua/project" style="text-decoration: none">Project</a></html>
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=='''我们的项目'''==
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[[Image:China.gif|15px]]中文版[[Team:Tsinghua#Tsinghua University|Back]]
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2010年清华大学iGEM项目的重点是开发新的生产方法使用抗体合成生物学。其目的是'''模拟抗体的选择和生产工艺细菌'''。
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传统方法生产的抗体，比如杂交瘤细胞等技术，是昂贵和费时。杂交瘤技术，对B-细胞库构建是通过实验动物，比如老鼠;使用流式细胞仪来选择一个特异性抗体，然后用杂交瘤细胞收获抗体。
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在这个项目中，我们期待在大肠杆菌细胞中用一种廉价高效的方法实现所有这些过程。整个项目可分为两个模块：
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====模块 I: 抗体库的构建====
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我们对大肠杆菌的基因组先进行了预修饰，对其插入了重组序列与I-Sel的酶切位点。然后，我们在质粒里插入两端有重组位点的目的片段（抗体可变区V,D,J），导入大肠杆菌，诱导后利用重组序列的交叉序列即可以重组。我们具体实验验证时，会利用荧光蛋白与抗性基因的筛选。
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====模块 II: 特异性抗体的筛选====
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实验设计主要包括利用抗原抗体结合，通过过滤方法进行筛选的“滤纸过滤筛选法”和应用抗原抗体结合后菌体间结合强度增加的性质设计的“振荡筛选法”。前者相当于利用过滤这种最常用和简便的分离方法达到了抗体分离的目的，而后者实现了用抗生素抗性这种微生物操作中最简便的筛选方法分离抗体的目的。
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<html>See Details @ <a href="https://2010.igem.org/Team:Tsinghua/project" style="text-decoration: none">Project</a></html>
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=='''О проекте'''==
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[[Image:Russia.gif|15px]]Русский[[Team:Tsinghua#Tsinghua University|Back]]
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Tsinghua iGEM 2010 Project is focused on developing a new Antibody Production Method using Synthetic Biology.
+
-
The Aim is to
+
-
''' Simulate Antibody Selection and Production Technology with Bacteria'''.
+
-
 
+
-
Traditional method of antibody production such as Hybridoma Technology is expensive and time consuming. In Hybridoma Technology, generation of B-Cell library is accomplished inside experimental animal, mouse for example; using Flow Cytometry to select a specific kind of antibody; then hybridoma would be prepared to harvest antibody.
+
-
 
+
-
In this project, we expect to achieve all these processes cheap and efficiently in E.coli Cell. The whole project can be divided into two modules:
+
-
 
+
-
 
+
-
====MODULE I: Generation of Antibody Library====
+
-
 
+
-
====MODULE II: Selection of Specific Antibody====
+
-
 
+
-
<html>See Details @ <a href="https://2010.igem.org/Team:Tsinghua/project" style="text-decoration: none">Project</a></html>
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=='''Notre Projet'''==
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[[Image:France.gif|15px]]Français[[Team:Tsinghua#Tsinghua University|Back]]
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Tsinghua iGEM 2010 Project is focused on developing a new Antibody Production Method using Synthetic Biology.
+
-
The Aim is to
+
-
''' Simulate Antibody Selection and Production Technology with Bacteria'''.
+
-
 
+
-
Traditional method of antibody production such as Hybridoma Technology is expensive and time consuming. In Hybridoma Technology, generation of B-Cell library is accomplished inside experimental animal, mouse for example; using Flow Cytometry to select a specific kind of antibody; then hybridoma would be prepared to harvest antibody.
+
-
 
+
-
In this project, we expect to achieve all these processes cheap and efficiently in E.coli Cell. The whole project can be divided into two modules:
+
-
 
+
-
 
+
-
====MODULE I: Generation of Antibody Library====
+
-
 
+
-
====MODULE II: Selection of Specific Antibody====
+
-
 
+
-
<html>See Details @ <a href="https://2010.igem.org/Team:Tsinghua/project" style="text-decoration: none">Project</a></html>
+
-
 
+
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=='''Unser Projekt'''==
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[[Image:Germany.gif|15px]]Deutsch[[Team:Tsinghua#Tsinghua University|Back]]
+
-
 
+
-
Tsinghua iGEM 2010 Project is focused on developing a new Antibody Production Method using Synthetic Biology.
+
-
The Aim is to
+
-
''' Simulate Antibody Selection and Production Technology with Bacteria'''.
+
-
 
+
-
Traditional method of antibody production such as Hybridoma Technology is expensive and time consuming. In Hybridoma Technology, generation of B-Cell library is accomplished inside experimental animal, mouse for example; using Flow Cytometry to select a specific kind of antibody; then hybridoma would be prepared to harvest antibody.
+
-
 
+
-
In this project, we expect to achieve all these processes cheap and efficiently in E.coli Cell. The whole project can be divided into two modules:
+
-
 
+
-
 
+
-
====MODULE I: Generation of Antibody Library====
+
-
 
+
-
====MODULE II: Selection of Specific Antibody====
+
-
 
+
-
<html>See Details @ <a href="https://2010.igem.org/Team:Tsinghua/project" style="text-decoration: none">Project</a></html>
+
-
 
+
-
=='''Nuestro Proyecto'''==
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[[Image:Spain.gif|15px]]Español  [[Team:Tsinghua#Tsinghua University|Back]]
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-
 
+
-
Tsinghua iGEM 2010 Project is focused on developing a new Antibody Production Method using Synthetic Biology.
+
-
The Aim is to
+
-
''' Simulate Antibody Selection and Production Technology with Bacteria'''.
+
-
 
+
-
Traditional method of antibody production such as Hybridoma Technology is expensive and time consuming. In Hybridoma Technology, generation of B-Cell library is accomplished inside experimental animal, mouse for example; using Flow Cytometry to select a specific kind of antibody; then hybridoma would be prepared to harvest antibody.
+
-
 
+
-
In this project, we expect to achieve all these processes cheap and efficiently in E.coli Cell. The whole project can be divided into two modules:
+
-
 
+
-
 
+
-
====MODULE I: Generation of Antibody Library====
+
-
 
+
-
====MODULE II: Selection of Specific Antibody====
+
-
 
+
-
 
+
-
=='''私たちのプロジェクト'''==
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-
[[Image:Japan.gif|15px]]日本語  [[Team:Tsinghua#Tsinghua University|Back]]
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清華せいさつ2010プロジェクトは、新しい抗体の生産方法は、合成生物学を使用して開発に焦点を当てています。
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目的には
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'''をシミュレート抗体の選択と生産技術細菌です'''。
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ハイブリドーマ技術などの抗体産生の伝統的な方法は、コストと時間がかかります。ハイブリドーマ技術では、B細胞ライブラリの生成は実験動物内で、たとえばマウスが、抗体の特定の種類を選択するフローサイトメトリーを使用して、次にハイブリドーマは、収穫抗体を準備される実行されます。
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このプロジェクトでは、我々は安く、大腸菌細胞を効率的に処理するすべてのこれらを実現する予定です。プロジェクト全体は、2つのモジュールに分割することができます：
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====モジュール I: 建設ののライブラリ====
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====モジュール II: フィルタの特定の抗体====
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Latest revision as of 15:41, 21 October 2010

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Team

We're from Tsinghua University.

Since China opened up to the world in 1978, Tsinghua University has developed at a breathtaking pace into a comprehensive research university. At present, the university has 14 schools and 56 departments with faculties in science, engineering, humanities, law, medicine, history, philosophy, economics, management, education and art. The University has now over 25,900 students, including 13,100 undergraduates and 12,800 graduate students. As one of China’s most renowned universities, Tsinghua has become an important institution for fostering talent and scientific research.

With the motto of “Self-Discipline and Social Commitment” and the spirit of “Actions Speak Louder than Words”, Tsinghua University is dedicated to the well-being of Chinese society and to world development.

More...

Project

Monoclonal antibodies (mAb or moAb) are monospecific antibodies that are the same, made by identical immune cells that are all clones of a unique parent cell. When stimulated by almost any type of antigen, the immune system can create the specific antibody. This lays the foundation for monoclonal artificial antibodies.

The current most well-developed technique in Artificial Monoclonal Antibody is the famous Hybridoma Cell Production. Monoclonal antibodies are typically made by fusing myeloma cells with the spleen cells from a mouse that has been immunized with the desired antigen. The success rate is so low that a selective medium in which only fused cells can grow is used. This mixture of cells is then diluted and clones are grown from single parent cells on microtitre wells. The antibodies secreted by the different clones are then assayed for their ability to bind to the antigen. The most productive and stable clone is then selected for future use.

More...

Parts

We built a host of parts during our project. The idea is that after every small step, we store our sequence as a biobrick part.

This strategy marks our progress and facilitates future use of these sequences.

More...

Experiment

Our experiments are carefully recorded on a daily basis. Through the series of records, we can see our joys and sorrows.

Besides, we made the records for the purpose that our experiments can be repeated one day by someone else, thus contributing to the exploration of the unknown.

More...

Support

Our project is supported by School of Life Sciences, Department of Physics, Academy of Arts and Designs in Tsinghua.

Besides, we cited from a series of references.

More...

Human Practice

We put safety as our first priority and made a detailed safety brochure.

We also devoted efforts to publicize synthetic biology and to cooperate with other teams. The teams in China held a summer meetup to discuss our progress and share our resources. We also held a lecture introducing iGEM and our project in Tsinghua Univeristy. To get further support, we sought the cooperation of iGEM Team at Macquire, Australia.

More...

Retrieved from "http://2010.igem.org/Team:Tsinghua"