Team:SDU-Denmark/primers
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On this page we list all the primers we designed and used during the summer. All primers were kindly provided by [http://www.dna-technology.dk/ DNA Technology]. <br><br> | On this page we list all the primers we designed and used during the summer. All primers were kindly provided by [http://www.dna-technology.dk/ DNA Technology]. <br><br> | ||
- | <table style="text-align: left;" border="1" | + | |
- | + | <!DOCTYPE html PUBLIC "-//W3C//DTD HTML 4.01//EN" "http://www.w3.org/TR/html4/strict.dtd"> | |
+ | <html> | ||
+ | <head> | ||
+ | </head> | ||
+ | <body> | ||
+ | <table style="text-align: left; width: 630px; height: 508px;" | ||
+ | border="1" cellpadding="2" cellspacing="2"> | ||
+ | <tbody> | ||
<tr> | <tr> | ||
- | <td>Primer name</td> | + | <td |
- | <td | + | style="width: 137px; font-family: Helvetica,Arial,sans-serif; height: 44px;">Primer |
- | + | name</td> | |
- | <td | + | <td |
- | + | style="width: 414px; font-family: Helvetica,Arial,sans-serif; height: 44px;">Sequence</td> | |
+ | <td | ||
+ | style="width: 51px; font-family: Helvetica,Arial,sans-serif; height: 44px;">Tm | ||
+ | (C)</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td> | + | <td |
- | <td>5'- | + | style="width: 137px; font-family: Helvetica,Arial,sans-serif; height: 44px;">FlhDC |
- | + | fw</td> | |
- | + | <td | |
- | <td>88.3</td> | + | style="width: 414px; font-family: Courier New; height: 44px;">5'-GTTTCTTC<span |
+ | style="color: rgb(80, 204, 56);">GAATTC</span>GCGGCCGCT<span | ||
+ | style="color: rgb(80, 204, 56);">TCTAG</span><span | ||
+ | style="color: rgb(88, 115, 248);"><span | ||
+ | style="color: rgb(255, 153, 0);">A</span>TGCATACCTCCGAGTTGCTGAAA</span>-3'</td> | ||
+ | <td | ||
+ | style="width: 51px; font-family: Helvetica,Arial,sans-serif; height: 44px;">88.3</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td> | + | <td |
- | <td>5'- | + | style="width: 137px; font-family: Helvetica,Arial,sans-serif; height: 44px;">FlhDC |
- | + | rv</td> | |
- | + | <td | |
- | <td>88.3</td> | + | style="width: 414px; font-family: Courier New; height: 44px;">5'-GTTTCTTC<span |
+ | style="color: rgb(80, 204, 56);">CTGCAG</span>CGGCCGCTACTATT<span | ||
+ | style="color: rgb(88, 115, 248);">AAACAGCCTGTACTCTCTGTTC</span>-3'</td> | ||
+ | <td | ||
+ | style="width: 51px; font-family: Helvetica,Arial,sans-serif; height: 44px;">88.3</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td> | + | <td |
- | <td>5'- | + | style="width: 137px; font-family: Helvetica,Arial,sans-serif; height: 44px;">FlhDC |
- | + | mutation fw*</td> | |
- | + | <td | |
- | <td></td> | + | style="width: 414px; font-family: Courier New; height: 44px;">5'-<span |
+ | style="color: rgb(88, 115, 248);">TTGGCAGCTTTGCCCGCAGCTTATG</span>-3'</td> | ||
+ | <td | ||
+ | style="width: 51px; font-family: Helvetica,Arial,sans-serif; height: 44px;">61.0</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td> | + | <td |
- | <td>5'- | + | style="width: 137px; font-family: Helvetica,Arial,sans-serif; height: 44px;">FlhDC |
- | + | mutation rv*</td> | |
- | + | <td | |
- | <td></td> | + | style="width: 414px; font-family: Courier New; height: 44px;">5'-<span |
+ | style="color: rgb(88, 115, 248);">CATAAGCTGCGGGCAAAGCTGCCAA</span>-3'</td> | ||
+ | <td | ||
+ | style="width: 51px; font-family: Helvetica,Arial,sans-serif; height: 44px;">61.0</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>Photosensor | + | <td |
- | <td>5'- | + | style="width: 137px; font-family: Helvetica,Arial,sans-serif; height: 44px;">Photosensor |
- | + | fw</td> | |
- | + | <td | |
- | <td>73.1</td> | + | style="width: 414px; font-family: Courier New; height: 44px;">5'-GTTTCTTC<span |
+ | style="color: rgb(80, 204, 56);">GAATTC</span>GCGGCCGCT<span | ||
+ | style="color: rgb(80, 204, 56);">TCTAG</span><span | ||
+ | style="color: rgb(88, 115, 248);"><span | ||
+ | style="color: rgb(255, 102, 0);">A</span>TGGTGGGACTTACGACCTC</span>-3'</td> | ||
+ | <td | ||
+ | style="width: 51px; font-family: Helvetica,Arial,sans-serif; height: 44px;">73.1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td>Photosensor | + | <td |
- | <td>5'- | + | style="width: 137px; font-family: Helvetica,Arial,sans-serif; height: 44px;">Photosensor |
- | + | rv</td> | |
- | + | <td | |
- | <td>73.9</td> | + | style="width: 414px; font-family: Courier New; height: 44px;">5'-GTTTCTTC<span |
+ | style="color: rgb(80, 204, 56);">CTGCAG</span>CGGCCGCT<span | ||
+ | style="color: rgb(80, 204, 56);">ACTAGT</span>A<span | ||
+ | style="color: rgb(88, 115, 248);">TCAGAAGGTTTCCCAGTTCGCATC</span>-3'</td> | ||
+ | <td | ||
+ | style="width: 51px; font-family: Helvetica,Arial,sans-serif; height: 44px;">73.9</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td | + | <td |
- | <td>5'- | + | style="width: 137px; font-family: Helvetica,Arial,sans-serif; height: 44px;">NinaB |
- | + | fw</td> | |
- | + | <td | |
- | <td>73.1</td> | + | style="width: 414px; font-family: Courier New; height: 44px;">5'-<span |
+ | style="color: rgb(80, 204, 56);"><span | ||
+ | style="color: black;">GTTTCTTC</span>GAATTC</span>GCGGCCGCT<span | ||
+ | style="color: rgb(80, 204, 56);">TCTAG</span><span | ||
+ | style="color: rgb(88, 115, 248);"><span | ||
+ | style="color: rgb(255, 102, 0);">A</span>TGGCAGCCGGTGTCTTCAAGAG</span>-3'</td> | ||
+ | <td | ||
+ | style="width: 51px; font-family: Helvetica,Arial,sans-serif; height: 44px;">73.1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td | + | <td |
- | <td>5'- | + | style="width: 137px; font-family: Helvetica,Arial,sans-serif; height: 44px;">NinaB |
- | + | rv</td> | |
- | + | <td | |
- | <td>72.4</td> | + | style="width: 414px; font-family: Courier New; height: 44px;">5'-<span |
+ | style="color: rgb(80, 204, 56);"><span | ||
+ | style="color: black;">GTGTCTTC</span>CTGCAG</span>CGGCCGCT<span | ||
+ | style="color: rgb(80, 204, 56);">ACTAGT</span>A<span | ||
+ | style="color: rgb(88, 115, 248);">CTAAATGGCATTGGGTGCAAACCATC</span>-3'</td> | ||
+ | <td | ||
+ | style="width: 51px; font-family: Helvetica,Arial,sans-serif; height: 44px;">72.4</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td | + | <td |
- | <td>5'- | + | style="width: 137px; font-family: Helvetica,Arial,sans-serif; height: 44px;">NinaB |
- | + | 2 fw**</td> | |
- | + | <td | |
- | <td>68.1</td> | + | style="width: 414px; font-family: Courier New; height: 44px;">5'-CCGCT<span |
+ | style="color: rgb(80, 204, 56);">TCTAG</span><span | ||
+ | style="color: rgb(88, 115, 248);"><span | ||
+ | style="color: rgb(255, 102, 0);">A</span>TGGCAGCCGGTGTCTTCAAGAG</span>-3'</td> | ||
+ | <td | ||
+ | style="width: 51px; font-family: Helvetica,Arial,sans-serif; height: 44px;">68.1</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
- | <td | + | <td |
- | <td>5'- | + | style="width: 137px; font-family: Helvetica,Arial,sans-serif; height: 44px;">NinaB |
- | + | 2 rv**</td> | |
- | + | <td | |
- | <td>65.6</td> | + | style="width: 414px; font-family: Courier New; height: 44px;">5'-CGCT<span |
+ | style="color: rgb(80, 204, 56);">ACTAGT</span>A<span | ||
+ | style="color: rgb(88, 115, 248);">CTAAATGGCATTGGGTGCAAACCATC</span>-3'</td> | ||
+ | <td | ||
+ | style="width: 51px; font-family: Helvetica,Arial,sans-serif; height: 44px;">65.6</td> | ||
</tr> | </tr> | ||
- | </table><br> | + | </tbody> |
- | + | </table> | |
- | + | <br> | |
+ | </body> | ||
+ | </html> | ||
+ | |||
+ | |||
+ | (*) Mutation primers were designed to introduce a silent mutation at position 822 in the operon to eliminate an illegal PstI site.<br> | ||
+ | (**) Due to the formation of hairpin structures of primers ninaB fw and ninaB rv, new primers were designed containing only the innermost restriction sites (XbaI and SpeI).<br><br> | ||
</p> | </p> |
Revision as of 13:31, 21 October 2010