Team:Minnesota/Protocols

From 2010.igem.org

(Difference between revisions)
Line 15: Line 15:
<h2>Ligase Free Cloning</h2>
<h2>Ligase Free Cloning</h2>
-
This technique
+
This technique uses e. coli ligase to ligate a gene of interest with a plasmid backbone that both have compatible sticky ends.
 +
 
<b>Step 1</b>
<b>Step 1</b>

Revision as of 01:18, 21 October 2010

Mnlogo.jpg
Home Team Project Protocols Notebook Judging Criteria Safety

Protocols

In this section we will offer protocols for techniques we have used that are not part of comerically avalible kits.

Ligase Free Cloning

This technique uses e. coli ligase to ligate a gene of interest with a plasmid backbone that both have compatible sticky ends.


Step 1 Information

Step 2 Information.

Protocol 2

about

Step 1 Information

Step 2 Information.