Team:Imperial College London/Software Tool

From 2010.igem.org

(Difference between revisions)
Line 21: Line 21:
   det[5] = "The complement system is part of the innate immune response to an acute infection. Detecting C3 convertase could give medical professionals a rapid method of seeing if a patient is fighting an acute infection.";
   det[5] = "The complement system is part of the innate immune response to an acute infection. Detecting C3 convertase could give medical professionals a rapid method of seeing if a patient is fighting an acute infection.";
   var prefix = "GAATTCGCGGCCGCTTCTAG";
   var prefix = "GAATTCGCGGCCGCTTCTAG";
-
   var promoter = "AATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTAAATTTTATTTGACAAAAATGGGCTCGTGTTGTACAATAAATGT";
+
   var promoter = "AATTTTGTCAAAATAATTTTATTGACAACGTCTTATTAACGTTGATATAATTTAAATTTTATTTGAC AAAAATGGGCTCGTGTTGTACAATAAATGT";
   var rbs = "TACTAGAGTCTTCAGAAAGGAGT";
   var rbs = "TACTAGAGTCTTCAGAAAGGAGT";
   var scar = "blank";
   var scar = "blank";
Line 105: Line 105:
'''<span style="color:#D34649">Red</span> - Ribosome Binding Site'''
'''<span style="color:#D34649">Red</span> - Ribosome Binding Site'''
 +
 +
'''<span style="color:#FFFFFF">White</span> - Scar'''
'''<span style="color:#AB6197">Purple</span> - Cell Wall Binding Domain'''
'''<span style="color:#AB6197">Purple</span> - Cell Wall Binding Domain'''

Revision as of 11:39, 20 October 2010

Software Tool
We realised early on that our detection module could be designed with a sensitivity to different proteases. By changing the cleavage site the system can accept a wide variety of inputs. This tool is designed to facilitate a quick custom sequence generation of the entire surface protein construct.
Select Protease Description

This was our primary target. Read our wiki to find out more!

Awaiting sequence generation...

Yellow - Biobrick Prefix/Suffix

Orange - Promoter

Red - Ribosome Binding Site

White - Scar

Purple - Cell Wall Binding Domain

Dark Blue - Adjustable Linker

Light Blue - Protease Cleavage Site

Dark Green - Autoinducing Peptide

Light Green - Terminator