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Team:Imperial College London/Software Tool
From 2010.igem.org
(Difference between revisions)
| Line 14: | Line 14: | ||
<script type="text/javascript"> | <script type="text/javascript"> | ||
$(document).ready(function(){ | $(document).ready(function(){ | ||
| - | var det = | + | var det = new Array(); |
det[1] = "This was our primary target. Read our wiki to find out more!"; | det[1] = "This was our primary target. Read our wiki to find out more!"; | ||
det[2] = "test2"; | det[2] = "test2"; | ||
| Line 20: | Line 20: | ||
det[4] = "test4"; | det[4] = "test4"; | ||
det[5] = "test5"; | det[5] = "test5"; | ||
| + | var prefix = "blank"; | ||
| + | var promoter = "blank"; | ||
| + | var rbs = "TACTAGAGTCTTCAGAAAGGAGT"; | ||
| + | var cwb = "ATGCGTTCTTATATAAAAGTCCTAACAATGTGTTTTCTGGGGCTCATACTTTTTGTGCCAACAGCTTTGGCCGATAACTCAGTGAAAAGAGTTGGGGGAAGCAATAGATACGGCACTGCTGTACAAATATCAAAGCAAATGTATTCAACAGCAAGTACAGCTGTAATTGTTGGTGGGAGTTCCTATGCAGATGCTATTTCAGCAGCACCTCTTGCTTACCAGAAGAATGCGCCATTGCTTTACACTAATTCTGATAAGCTTTCATATGAAACGAAAACAAGATTGAAAGAGATGCAGACTAAAAATGTAATTATTGTAGGCGGAACACCTGCTGTTTCTTCTAACACTGCTAACCAGATTAAAAGCTTGGGGATAAGTATTAAACGAATTGCAGGAAGCAACCGTTATGATACGGCTGCACGGGTGGCAAAAGCGATGGGTGCGACTTCAAAAGCTGTTATTTTGAACGGCTTCTTATATGCAGACGCTCCGGCCGTCATCCCTTATGCAGCGAAAAACGGGTATCCAATTCTTTTTACAAATAAAACATCTATAAATAGTGCGACTACGTCTGTGATAAAAGATAAGGGAATTTCGAGTACCGTTGTTGTAGGAGGCACTGGAAGTATCAGCAATACGGTATACAACAAGTTACCTTCTCCTACAAGAATTAGCGGTTCAAACAGATATGAGCTTGCTGCAAATATCGTACAAAAACTTAATTTATCAACAAGCACCGTATATGTAAGCAATGGATTCAGCTACCCTGACTCTATTGCAGGAGCTACACTGGCAGCTAAGAAGAAGCAATCTCTTATTCTTACAAATGGTGAAAATTTATCTACAGGAGCCCGTAAAATTATTGGAAGTAAAAACATGTCAAACTTTATGATTATCGGAAACACTCCTGCCGTAAGCACAAAGGTTGCTAATCAGCTAAAGAATCCAGTTGTA"; | ||
| + | var linker = "AGCAGGGGTAGCAGGGCTGGTGGCGGTGGCGGTGGC"; | ||
| + | var cleavage = new Array(); | ||
| + | cleavage[1] = "AGCTGGCCTCTT"; | ||
| + | cleavage[2] = "blank"; | ||
| + | cleavage[3] = "blank"; | ||
| + | cleavage[4] = "blank"; | ||
| + | cleavage[5] = "blank"; | ||
| + | var aip = "GAAATGCGCCTTAGCAAATTCTTCAGGGACTTCATTCTTCAAAGGAAAAAA"; | ||
| + | var terminator = "TAATAA"; | ||
| + | var suffix = "blank"; | ||
| + | |||
$("#paraselect").change(function() | $("#paraselect").change(function() | ||
{ | { | ||
| Line 29: | Line 44: | ||
if (message_index > 0) | if (message_index > 0) | ||
$("#parades").append(det[message_index]); | $("#parades").append(det[message_index]); | ||
| - | }); | + | }); |
| + | |||
| + | $('#parago').click(function() { | ||
| + | $("#sequenceout").empty(); | ||
| + | $("#spre").append(prefix); | ||
| + | $("#spro").append(promoter); | ||
| + | $("#srbs").append(rbs); | ||
| + | $("#scwb").append(cwb); | ||
| + | $("#slin").append(linker); | ||
| + | $("#scle").append(cleavage[message_index]); | ||
| + | $("#saip").append(aip); | ||
| + | $("#ster").append(terminator); | ||
| + | $("#ssuf").append(suffix); | ||
| + | }); | ||
}); | }); | ||
</script> | </script> | ||
| Line 64: | Line 92: | ||
|- | |- | ||
|style="width:860px;background:#d5d5d5;border: solid 20px #d5d5d5;" colspan="3"|'''<html> | |style="width:860px;background:#d5d5d5;border: solid 20px #d5d5d5;" colspan="3"|'''<html> | ||
| - | Awaiting sequence generation... | + | <div id="sequenceout">Awaiting sequence generation... <span id="spre" style="color:#F6CF39"></span><span id="spro" style="color:#FC8E42"></span><span id="srbs" style="color:#D34649"></span><span id="scwb" style="color:#AB6197"></span><span id="slin" style="color:#6273A8"></span><span id="scle" style="color:#6A9ECD"></span><span id="saip" style="color:#68904E"></span><span id="ster" style="color:#9DB742"></span><span id="ssuf" style="color:#F6CF39"></span></div> |
</html>''' | </html>''' | ||
|- | |- | ||
Revision as of 09:47, 20 October 2010
| Software Tool |
| We realised early on that our detection module could be designed with a sensitivity to different proteases. By changing the cleavage site the system can accept a wide variety of inputs. This tool is designed to facilitate a quick custom sequence generation of the entire surface protein construct. |
| Select Protease | Description | |
|
This was our primary target. Read our wiki to find out more! | ||
|
Awaiting sequence generation...
| ||
|
Yellow - Biobrick Prefix/Suffix Orange - Promoter Red - Ribosome Binding Site Purple - Cell Wall Binding Domain Dark Blue - Adjustable Linker Light Blue - Protease Cleavage Site Dark Green - Autoinducing Peptide Light Green - Terminator | ||
