Team:KAIST-Korea/Notebook/Diary/September
From 2010.igem.org
(Difference between revisions)
(→September 2 ~ September 28) |
|||
(134 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
__NOTOC__ | __NOTOC__ | ||
{{:Team:KAIST/header}} | {{:Team:KAIST/header}} | ||
- | <table width="100%" border="0"> | + | <table width="100%" border="0" cellpadding = "20px"> |
<tr> | <tr> | ||
Line 9: | Line 9: | ||
<table width="100%"> | <table width="100%"> | ||
<tr> | <tr> | ||
- | <td> | + | <td align=center > |
<html><a href="https://2010.igem.org/Team:KAIST-Korea/Notebook/Diary/April"><img src="https://static.igem.org/mediawiki/2010/e/e1/April.jpg" width=100></a></html> | <html><a href="https://2010.igem.org/Team:KAIST-Korea/Notebook/Diary/April"><img src="https://static.igem.org/mediawiki/2010/e/e1/April.jpg" width=100></a></html> | ||
</td> | </td> | ||
- | <td> | + | <td align=center > |
<html><a href="https://2010.igem.org/Team:KAIST-Korea/Notebook/Diary/May"><img src="https://static.igem.org/mediawiki/2010/6/6d/5%EC%9B%94.jpg | <html><a href="https://2010.igem.org/Team:KAIST-Korea/Notebook/Diary/May"><img src="https://static.igem.org/mediawiki/2010/6/6d/5%EC%9B%94.jpg | ||
" width=100></a></html> | " width=100></a></html> | ||
</td> | </td> | ||
- | <td> | + | <td align=center > |
<html><a href="https://2010.igem.org/Team:KAIST-Korea/Notebook/Diary/June"><img src=" https://static.igem.org/mediawiki/2010/3/31/6%EC%9B%94.jpg" width=100></a></html> | <html><a href="https://2010.igem.org/Team:KAIST-Korea/Notebook/Diary/June"><img src=" https://static.igem.org/mediawiki/2010/3/31/6%EC%9B%94.jpg" width=100></a></html> | ||
</td> | </td> | ||
- | <td> | + | <td align=center > |
<html><a href="https://2010.igem.org/Team:KAIST-Korea/Notebook/Diary/July"><img src="https://static.igem.org/mediawiki/2010/4/49/7%EC%9B%94.jpg | <html><a href="https://2010.igem.org/Team:KAIST-Korea/Notebook/Diary/July"><img src="https://static.igem.org/mediawiki/2010/4/49/7%EC%9B%94.jpg | ||
" width=100></a></html> | " width=100></a></html> | ||
</td> | </td> | ||
- | <td> | + | <td align=center > |
<html><a href="https://2010.igem.org/Team:KAIST-Korea/Notebook/Diary/August"><img src="https://static.igem.org/mediawiki/2010/5/55/8%EC%9B%94.jpg | <html><a href="https://2010.igem.org/Team:KAIST-Korea/Notebook/Diary/August"><img src="https://static.igem.org/mediawiki/2010/5/55/8%EC%9B%94.jpg | ||
" width=100></a></html> | " width=100></a></html> | ||
</td> | </td> | ||
- | <td> | + | <td align=center > |
<html><a href="https://2010.igem.org/Team:KAIST-Korea/Notebook/Diary/September"><img src="https://static.igem.org/mediawiki/2010/0/02/9%EC%9B%94.jpg | <html><a href="https://2010.igem.org/Team:KAIST-Korea/Notebook/Diary/September"><img src="https://static.igem.org/mediawiki/2010/0/02/9%EC%9B%94.jpg | ||
" width=100></a></html> | " width=100></a></html> | ||
</td> | </td> | ||
- | <td> | + | <td align=center > |
<html><a href="https://2010.igem.org/Team:KAIST-Korea/Notebook/Diary/October"><img src="https://static.igem.org/mediawiki/2010/9/91/10%EC%9B%94.jpg | <html><a href="https://2010.igem.org/Team:KAIST-Korea/Notebook/Diary/October"><img src="https://static.igem.org/mediawiki/2010/9/91/10%EC%9B%94.jpg | ||
" width=100></a></html> | " width=100></a></html> | ||
</td> | </td> | ||
- | <td> | + | <td align=center > |
<html><a href="https://2010.igem.org/Team:KAIST-Korea/Notebook/Diary/November"><img src="https://static.igem.org/mediawiki/2010/3/35/11%EC%9B%94.jpg | <html><a href="https://2010.igem.org/Team:KAIST-Korea/Notebook/Diary/November"><img src="https://static.igem.org/mediawiki/2010/3/35/11%EC%9B%94.jpg | ||
" width=100></a></html> | " width=100></a></html> | ||
Line 44: | Line 44: | ||
</table> | </table> | ||
+ | == September 1 == | ||
+ | pREP41 vector has arrived. | ||
+ | -> we will do E.coli transformation and increase its number. | ||
- | + | pREP42-GFP vector has arrived (in E.coli) | |
- | </ | + | -> will incubate a day and do spreading for further experiment. |
+ | |||
+ | Making culture media | ||
+ | # 500mL LB broth + agar 1.5% (7.5g) | ||
+ | # Do auto clave | ||
+ | # Cool it down until it reaches 50~60℃ | ||
+ | # Add ampicillin(1000x 100mg/mL) and stir | ||
+ | # putting out bubbles on culture media | ||
+ | # pouling on petri dish and wait for 1 hour | ||
+ | |||
+ | Culture | ||
+ | # Competent cell + DN10A and leave it in ice for 30min | ||
+ | # Do heat shock for 45 sec and store it in ice for 2min | ||
+ | # Stabilize it in incubator for 30 to 40min | ||
+ | # Do spreading | ||
+ | |||
+ | |||
+ | ==September 2 ~ September 21 == | ||
+ | <html> | ||
+ | We designed PCR schedules and do PCR experiment. When PCR result was not satisfied, then we repeated PCR experiment. There are PCR details on <a href=https://2010.igem.org/Team:KAIST-Korea/Project/Methods/PCR_Result>PCR experiment menu</a>. | ||
+ | </html> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> |
Latest revision as of 07:21, 20 October 2010
September 1pREP41 vector has arrived. -> we will do E.coli transformation and increase its number. pREP42-GFP vector has arrived (in E.coli) -> will incubate a day and do spreading for further experiment. Making culture media
Culture
September 2 ~ September 21
We designed PCR schedules and do PCR experiment. When PCR result was not satisfied, then we repeated PCR experiment. There are PCR details on PCR experiment menu.
|