Team:SDU-Denmark/project-i

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== Retinal Biosynthesis ==
== Retinal Biosynthesis ==
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For the photoreceptor to work, we will need to supply it with retinal. Therefor we will be building on work done in the Cambridge 2009 project, that introduced beta-carotene synthesis in E. coli, and contribute an enzyme that will catalyze cleavage of beta-carotene into retinal. By this our biological machine can supply it self with everything it needs to function, so that once the system has been established, there is need to intervene (except for the light regulation).<br>
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For the photoreceptor to work, we will need to supply it with enzymes for retinal biosynthesis. Retinal is formed by cleaving Beta-carotene, a reaction that is catalyzed by Beta-carotene-oxygenases. We will be supplying a new biobrick that expresses a Beta-carotene 15'15-monooxygenase from Drosophila Melanogaster. Beta-carotene biosynthesis will be supplied by a part made by the Cambridge 2009 team. We Have also done further characterisation of the Cambridge part in new strains and with different analytical methods.
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On top of creating a microfluidic flow generator, we will hopefully at the same time create a system that can mix fluids in microtubes. It is often a problem when working with tubes of this dimension, that if you let two liquids flow into them, they will not mix. The turbulence created by the bacteria's flagella will make both liquids move around randomly in the tube, so that they will become one in the end. <br>
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= Prospects =
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On top of creating a microfluidic flow generator, we will hopefully at the same time create a system that can mix fluids in microtubes. It is often a problem when working in nano-scale spaces that if you let two liquids flow into them, they will not mix. The turbulence created by the bacteria's flagella will make both liquids move around randomly in the tube, so that they will become one in the end. <br><br>
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The plan is that these three subprojects will result in a biobrick each:<br>
The plan is that these three subprojects will result in a biobrick each:<br>
- A constitutively active operon with the master regulator of flagella synthesis.<br>
- A constitutively active operon with the master regulator of flagella synthesis.<br>
- A bluelight photosensor coupled to the chemotaxis pathway.<br>
- A bluelight photosensor coupled to the chemotaxis pathway.<br>
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- A generator for the enyzme that cleaves beta-carotene to retinal.<br>
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- A generator for the enyzme that cleaves beta-carotene to retinal.<br><br>
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== References ==
== References ==

Revision as of 22:47, 18 October 2010