Team:Edinburgh/BioBricks

From 2010.igem.org

(Difference between revisions)
Line 174: Line 174:
   <td><a href="http://partsregistry.org/Part:BBa_K322706">BBa_K322706</a></td>
   <td><a href="http://partsregistry.org/Part:BBa_K322706">BBa_K322706</a></td>
   <td>Downstream region of <i>E. coli</i> tryptophanase locus, used for targetting genes to this locus using BRIDGE.</td>
   <td>Downstream region of <i>E. coli</i> tryptophanase locus, used for targetting genes to this locus using BRIDGE.</td>
 +
</tr>
 +
<tr>
 +
  <td align="left"><b></b></td>
 +
  <td>Composite</td>
 +
  <td><a href="http://partsregistry.org/Part:BBa_K322922">BBa_K322922</a></td>
 +
  <td></td>
  </tr>
  </tr>
</table>
</table>
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   <th width="100px"><u>Link</u></th>
   <th width="100px"><u>Link</u></th>
   <th><u>Description</u></th>
   <th><u>Description</u></th>
 +
</tr>
 +
<tr>
 +
  <td align="left"><b>Firefly luciferase S284T mutant</b></td>
 +
  <td>Coding</td>
 +
  <td><a href="http://partsregistry.org/Part:BBa_K322246">BBa_K322246</a></td>
 +
  <td>Firefly luciferase from <i>Photinus pyralis</i> mutated towards the red spectrum.</td>
 +
</tr>
 +
<tr>
 +
  <td align="left"><b></b></td>
 +
  <td>Composite</td>
 +
  <td><a href="http://partsregistry.org/Part:BBa_K322247">BBa_K322247</a></td>
 +
  <td></td>
 +
</tr>
 +
<tr>
 +
  <td align="left"><b>Firefly luciferase 356K mutant</b></td>
 +
  <td>Coding</td>
 +
  <td><a href="http://partsregistry.org/Part:BBa_K322211">BBa_K322211</a></td>
 +
  <td>Firefly luciferase S284T mutant from <i>Photinus pyralis</i> re-mutated towards the red spectrum.</td>
 +
</tr>
 +
<tr>
 +
  <td align="left"><b></b></td>
 +
  <td>Composite</td>
 +
  <td><a href="http://partsregistry.org/Part:BBa_K322212">BBa_K322212</a></td>
 +
  <td></td>
 +
</tr>
 +
<tr>
 +
  <td align="left"><b>Firefly luciferase</b></td>
 +
  <td>Coding</td>
 +
  <td><a href="http://partsregistry.org/Part:BBa_K322237">BBa_K322237</a></td>
 +
  <td>Ljubljana 2007's <a href="http://partsregistry.org/Part:BBa_I712019">BBa_I712019</a> in pSB1C3, from <i>Photinus pyralis</i>.</td>
 +
</tr>
 +
<tr>
 +
  <td align="left"><b></b></td>
 +
  <td>Composite</td>
 +
  <td><a href="http://partsregistry.org/Part:BBa_K322238">BBa_K322238</a></td>
 +
  <td></td>
 +
</tr>
 +
<tr>
 +
  <td align="left"><b>Codon optimised bright firefly luciferase</b></td>
 +
  <td>Coding</td>
 +
  <td><a href="http://partsregistry.org/Part:BBa_K322451">BBa_K322451</a></td>
 +
  <td>Firefly luciferase from <i>Photinus pyralis</i> mutated for increased bioluminescence.</td>
  </tr>
  </tr>
  <tr>
  <tr>
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   <td><a href="http://partsregistry.org/Part:BBa_K322007">BBa_K322007</a></td>
   <td><a href="http://partsregistry.org/Part:BBa_K322007">BBa_K322007</a></td>
   <td>Lumazine protein to shift the wavelength of LuxAB to blue.</td>
   <td>Lumazine protein to shift the wavelength of LuxAB to blue.</td>
 +
</tr>
 +
<tr>
 +
  <td align="left"><b></b></td>
 +
  <td>Composite</td>
 +
  <td><a href="http://partsregistry.org/Part:BBa_K322140">BBa_K322140</a></td>
 +
  <td></td>
 +
</tr>
 +
<tr>
 +
  <td align="left"><b></b></td>
 +
  <td>Composite</td>
 +
  <td><a href="http://partsregistry.org/Part:BBa_K322141">BBa_K322141</a></td>
 +
  <td></td>
 +
</tr>
 +
<tr>
 +
  <td align="left"><b></b></td>
 +
  <td>Signalling</td>
 +
  <td><a href="http://partsregistry.org/Part:BBa_K322149">BBa_K322149</a></td>
 +
  <td></td>
 +
</tr>
 +
<tr>
 +
  <td align="left"><b></b></td>
 +
  <td>Composite</td>
 +
  <td><a href="http://partsregistry.org/Part:BBa_K322150">BBa_K322150</a></td>
 +
  <td></td>
  </tr>
  </tr>
  <tr>
  <tr>
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  </tr>
  </tr>
  <tr>
  <tr>
-
   <td align="left"><b>Firefly luciferase</b></td>
+
   <td align="left"><b></b></td>
-
   <td>Coding</td>
+
   <td>Composite</td>
-
   <td><a href="http://partsregistry.org/Part:BBa_K322237">BBa_K322237</a></td>
+
   <td><a href="http://partsregistry.org/Part:BBa_K322999">BBa_K322999</a></td>
-
   <td>Ljubljana 2007's <a href="http://partsregistry.org/Part:BBa_I712019">BBa_I712019</a> in pSB1C3, from <i>Photinus pyralis</i>.</td>
+
   <td></td>
-
</tr>
+
-
<tr>
+
-
  <td align="left"><b>Firefly luciferase S284T mutant</b></td>
+
-
  <td>Coding</td>
+
-
  <td><a href="http://partsregistry.org/Part:BBa_K322246">BBa_K322246</a></td>
+
-
  <td>Firefly luciferase from <i>Photinus pyralis</i> mutated towards the red spectrum.</td>
+
-
</tr>
+
-
<tr>
+
-
  <td align="left"><b>Firefly luciferase 356K mutant</b></td>
+
-
  <td>Coding</td>
+
-
  <td><a href="http://partsregistry.org/Part:BBa_K322211">BBa_K322211</a></td>
+
-
  <td>Firefly luciferase S284T mutant from <i>Photinus pyralis</i> re-mutated towards the red spectrum.</td>
+
-
</tr>
+
-
<tr>
+
-
  <td align="left"><b>Codon optimised bright firefly luciferase</b></td>
+
-
  <td>Coding</td>
+
-
  <td><a href="http://partsregistry.org/Part:BBa_K322451">BBa_K322451</a></td>
+
-
  <td>Firefly luciferase from <i>Photinus pyralis</i> mutated for increased bioluminescence.</td>
+
  </tr>
  </tr>
</table>
</table>

Revision as of 16:44, 18 October 2010







BioBricks: Genomic BRIDGEs


For this part of the project we will have one main BioBrick, which will be the construct required for the two-step markerless insertion, encompassing cat (chloramphenicol resistance) and sacB (prevents growth on sucrose).

Chloramphenicol resistance has already been well characterised in the registry, so our characterisation has mainly focused on sacB.

In addition we will probably be submitting the construct with the up- and down- stream sequences of useful genes which can be removed, e.g. tnaA, which produces indole - when removed E. coli no longer smells!


Name Type Link Description
cat Translational Unit BBa_K322210 Chloramphenicol acetyltransferase (chloramphenicol resistance gene).
sacB Coding BBa_K322921 Bacillus subtilis levansucrase, lethal to E. coli in presence of sucrose.
tnaA upstream region Protein Domain BBa_K322705 Upstream region of E. coli tryptophanase locus, used for targetting genes to this locus using BRIDGE.
tnaA downstream region Protein Domain BBa_K322706 Downstream region of E. coli tryptophanase locus, used for targetting genes to this locus using BRIDGE.
Composite BBa_K322922

Composite BioBricks in preparation.





BioBricks: Bacterial BRIDGEs


***


Name Type Link Description
Firefly luciferase S284T mutant Coding BBa_K322246 Firefly luciferase from Photinus pyralis mutated towards the red spectrum.
Composite BBa_K322247
Firefly luciferase 356K mutant Coding BBa_K322211 Firefly luciferase S284T mutant from Photinus pyralis re-mutated towards the red spectrum.
Composite BBa_K322212
Firefly luciferase Coding BBa_K322237 Ljubljana 2007's BBa_I712019 in pSB1C3, from Photinus pyralis.
Composite BBa_K322238
Codon optimised bright firefly luciferase Coding BBa_K322451 Firefly luciferase from Photinus pyralis mutated for increased bioluminescence.
luxAB Translational Unit BBa_K322139 Bacterial luciferase producing blue light (from X. luminescens).
luxCDE Protein Domain BBa_K322312 Codes for a fatty acid reductase complex that makes the fatty acids necessary for LuxAB.
lumP Translational Unit BBa_K322007 Lumazine protein to shift the wavelength of LuxAB to blue.
Composite BBa_K322140
Composite BBa_K322141
Signalling BBa_K322149
Composite BBa_K322150
Phycocyanobilin synthesis operon Translational Unit BBa_K322122 Harvard 2008's BBa_K098010 in pSB1C3.
Phycocyanobilin synthesis operon without terminator Translational Unit BBa_K322123 BBa_K098010 without terminator, allowing addition of cph8.
cph8 with RBS Translational Unit BBa_K322124 Light sensing protein (chimeric Cph1 light receptor / EnvZ protein) for red light.
cph8 and reporter system (lacZ) Composite BBa_K322125 Red light sensor with lacZ reporter system.
cph8 and reporter system (EYFP) Composite BBa_K322126 Red light sensor with EYFP reporter system.
Phycocyanobilin synthesis genes with cph8 Composite BBa_K322127 The entire phycocyanobilin - cph8 system, without reporter system.
Phycocyanobilin synthesis genes with cph8 and EYFP reporter system Composite BBa_K322128 The entire phycocyanobilin - cph8 system, with EYFP reporter system.
Composite BBa_K322999

Composite BioBricks in preparation.




Retrieved from "http://2010.igem.org/Team:Edinburgh/BioBricks"