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Revision as of 11:37, 12 September 2010 (view source) Kschipper (Talk | contribs) (New page: =Characterization= Strains: * PalkS / AlkS: E.coli K12/310C * PalkS / AlkS / PalkB: E.coli K12/311C * P(CaiF) / AlkS / PalkB: E.coli K12/312C * Negative control: ''E.coli K12'' The ...) ← Older edit		Revision as of 15:34, 17 October 2010 (view source) Ebrinkman (Talk | contribs) (→Characterization) Newer edit →
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Revision as of 15:34, 17 October 2010

Characterization of the Sensing Parts

Strains:

• PalkS / AlkS: E.coli K12/310C
• PalkS / AlkS / PalkB: E.coli K12/311C
• P(CaiF) / AlkS / PalkB: E.coli K12/312C
• Negative control: E.coli K12

The characterization process of the hydrocarbon sensing sub-project will entail output measurements of fluorescence at varying octane concentrations (0.1% – 1%) of the above mentioned strains. These measurements will be performed using a 96-well plate reader. Growth curves will be followed by OD-600 alongside the fluorescence measurements. The obtained fluorescence and growth measurements will be analyzed and modeled accordingly.