Team:KAIST-Korea/Notebook/Diary/May

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Latest revision as of 05:15, 17 October 2010

May 26

Make spike and HNMT plasmid and transform bacteria with them. Bacteria expressing them will produce HNMT and engulf via membrane fusion. Ultimately, use the bacteria in a spray.

What we should do (June 1st~August 31th)

Syenthesis of bacteria
- Company: S.B. Yang>contact, rest>look for companies. Contact T.A.
- Sequence source: D.C. Yang, K.K. Kim
- 1),2): by June 1st
- KI building (Prof. Choi)
Homepage design
- K.K. Kim, +alpha
- SPARCS
- I.D. department
- 2),3): cost a lot - about \1million. stick with 1)
- S.B. Yang, K.K. Kim, D.C. Yang, H.S. Kim handle program until June 6th. The rest (design team) design.
Daily update
Confirmation of the product
- Florescent material such as GFP
- Quantum dot

May 27

1st solid idea: respiratory system using spike protein & HNMT
We have to make two plasmid, which would be a guide plasmid and a functional plasmid. We put spike gene in the guide plasmid and HNMT in the functional plasmid. Then, bacteria will get spikes on the outer membrane and HNMT inside the cell. After that, if we can pack HNMT in the vesicle, we can make a DDS. And we can check if HNMT works by using quantum dot or GFP.

Do list

See Stanford-secretion gene (everyone)
Find potential genes

Literature: H.S. Kim, S.H. Kim, S.J. Kim, Triple Yangs

Company: rest

by Saturday

KI workshop next Friday
DNA kit will arrive. Homepage design team assigned.
Potential name: FADEX (Fast Accurate Drug Exporting System)
Everyday 8 o'clock

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 == May 26 ==
-Make spike and HNMT plasmid and transform bacteria with it. Bacteria expressing it will produce HNMT and engulf via membrane fusion. Ultimately, use it as a spray.
+Make spike and HNMT plasmid and transform bacteria with them. Bacteria expressing them will produce HNMT and engulf via membrane fusion. Ultimately, use the bacteria in a spray.
+<br>
+<br>
 What we should do (June 1st~August 31th)
 # Syenthesis of bacteria
@@ Line 70: / Line 67: @@
 # Confirmation of the product
 #* Florescent material such as GFP
 #* Quantum dot
 == May 27 ==
-st solid idea: respiratory system using spike protein & HNMT
+st solid idea: respiratory system using spike protein & HNMT<br>
-We have to make two plasmid which would be guide plasmid and functional plasmid. We put spike gene in guide plasmid and HNMT in functional plasmid. Then bacteria will get spikes on outer membrane and HNMT inside the cell. After that if we can pack HNMT in the vesicle. Then we can make a DDS. And we can check if HNMT works by using quantum dot or GFP.
+We have to make two plasmid, which would be a guide plasmid and a functional plasmid. We put spike gene in the guide plasmid and HNMT in the functional plasmid. Then, bacteria will get spikes on the outer membrane and HNMT inside the cell. After that, if we can pack HNMT in the vesicle, we can make a DDS. And we can check if HNMT works by using quantum dot or GFP.
 : Do list
@@ Line 87: / Line 84: @@
 :* Everyday 8 o'clock
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