Team:Calgary/13 September 2010

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'''Monday September 13, 2010'''|
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Monday September 13, 2010|
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<u> Himika </u>
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[[Image:09.13.2010-Himika-male31+degp+i13507 rd.jpg|400px|thumb| Himika's restriction digest of DegP system with EcoRI/ SpeI]]
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Today I did a restriction digest of all the miniprepped colonies for DegP systems with EcoRI and SpeI. It looked like the construction worked but I will do a PCR of the plasmids before sending them off to sequencing.
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[[Image:09.13.2010-Himika-male31+degp+i13504 rd.jpg|400px|thumb| Himika's restriction digest of DegP system with EcoRI/ SpeI]]
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<u>Himika</u>
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Today I did a restriction digest of all the miniprepped colonies for DegP systems with EcoRI and SpeI. It looked like the construction worked but I will do a PCR of the plasmids before sending them off to sequencing. Today I also set up overnight cultures of MalE31-CpxR-RFP system. I also set up a restriction digest of MalE with XbaI/ PstI which will be constructed and transformed tomorrow with I0500-B0034.
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<u>Emily</u>
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Today I made overnight cultures of my malE31 colonies that I think might be biobricked and in the AK Vector.  I will miniprep these tomorrow and then proceed with a verification restriction digest.  Tonight I also helped Chris and Jeremy clean up some things in the lab.
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<u>Chris</u>
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Today, I helped Emily and Jeremy clean up the fume hood which had been previously contaminated with ethidium bromide. Yeah, that's a bad thing. We also tidied up the lab for the MDSC 408 class that will be sharing the lab with us under the supervision of our laboratory technician Deirdre Lobb.
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Latest revision as of 03:54, 17 October 2010

Monday September 13, 2010

Himika's restriction digest of DegP system with EcoRI/ SpeI
Himika's restriction digest of DegP system with EcoRI/ SpeI

Himika

Today I did a restriction digest of all the miniprepped colonies for DegP systems with EcoRI and SpeI. It looked like the construction worked but I will do a PCR of the plasmids before sending them off to sequencing. Today I also set up overnight cultures of MalE31-CpxR-RFP system. I also set up a restriction digest of MalE with XbaI/ PstI which will be constructed and transformed tomorrow with I0500-B0034.


Emily

Today I made overnight cultures of my malE31 colonies that I think might be biobricked and in the AK Vector. I will miniprep these tomorrow and then proceed with a verification restriction digest. Tonight I also helped Chris and Jeremy clean up some things in the lab.


Chris

Today, I helped Emily and Jeremy clean up the fume hood which had been previously contaminated with ethidium bromide. Yeah, that's a bad thing. We also tidied up the lab for the MDSC 408 class that will be sharing the lab with us under the supervision of our laboratory technician Deirdre Lobb.