O/N Culture

From 2010.igem.org

(Difference between revisions)
(New page: '''REMEMBER TO BE STERILE''' *Take 5mL LB and put in a round bottom tube *If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube '''MAKE SURE YOU ADD THE RIGHT ...)
 
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*Take 5mL LB and put in a round bottom tube
*Take 5mL LB and put in a round bottom tube
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*If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube '''MAKE SURE YOU ADD THE RIGHT ONE!'''
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*If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube  
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  *'''MAKE SURE YOU ADD THE RIGHT ONE!'''
*Take a tip on a p200 and lightly touch a colony
*Take a tip on a p200 and lightly touch a colony
*Eject the tip into the culture and put the cap on
*Eject the tip into the culture and put the cap on
*For a miniprep, grow O/N for 12-16 hours NOT LONGER
*For a miniprep, grow O/N for 12-16 hours NOT LONGER
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[http://openwetware.org/wiki/Bacterial_cell_culture]Openwetware
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Back to [[Team:Northwestern/Protocol|<span style="color:#4B088A;">Protocol</span>]]
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[[Category:Protocol]]

Latest revision as of 19:24, 16 October 2010

REMEMBER TO BE STERILE


  • Take 5mL LB and put in a round bottom tube
  • If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube
  *MAKE SURE YOU ADD THE RIGHT ONE!
  • Take a tip on a p200 and lightly touch a colony
  • Eject the tip into the culture and put the cap on
  • For a miniprep, grow O/N for 12-16 hours NOT LONGER

[http://openwetware.org/wiki/Bacterial_cell_culture]Openwetware

Back to Protocol