Team:SDU-Denmark/labnotes14

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<br>
<br>
Done By: Sheila and Louise<br>
Done By: Sheila and Louise<br>
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Date: 10/13 2010<br>
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Date: 11/10 2010<br>
Protocol: [https://2010.igem.org/Team:SDU-Denmark/protocols#TR1.1 TR1.1]<br>
Protocol: [https://2010.igem.org/Team:SDU-Denmark/protocols#TR1.1 TR1.1]<br>
Notes: <br>
Notes: <br>
Results: <br>
Results: <br>
Conclusion: <br>
Conclusion: <br>
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 +
== Retinal ==
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=== Miniprep of K343005+DT in PSB1AK3 ===
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Date: 30/09<br>
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Done by: Marie<br>
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Methods: Miniprep<br>
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Protocols: MP1.1[https://2010.igem.org/Team:SDU-Denmark/protocols#MP1.1]<br>
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Notes: <br>
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marie skriver dette...
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=== Restriction digest on K343002 and PSB1C3 ===
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Date: 12/10<br>
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Done by: Tommy<br>
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Methods: Restriction digest<br>
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Protocols: RD1.3[https://2010.igem.org/Team:SDU-Denmark/protocols#RD1.1]<br>
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Notes: <br>
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EcoRI and SpeI were used, the producted was purifided on a gel<br>
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nanodrop:<br>
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PSB1C3: 41,6<br>
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K343002: 64,5<br>
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=== Ligation of K343002 into PSB1C3 ===
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Date: 12/10<br>
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Done by: Marie & Christian<br>
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Methods: Ligation<br>
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Protocols: LG1.1[https://2010.igem.org/Team:SDU-Denmark/protocols#LG1.1]<br>
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Notes: <br>
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 +
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=== Transformations of K343002 in PSB1C3 into top10 E. Coli ===
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Date: 12/10<br>
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Done by: Tommy<br>
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Methods: Ligation<br>
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Protocols: LG1.1[https://2010.igem.org/Team:SDU-Denmark/protocols#LG1.1]<br>
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Notes: <br>
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16 colonies and RFP control was selected from the colonie PCR. All of the colonies contain K343002 in PSB1C3<br>
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VF2 and VR primers was used<br>
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PCR Program:<br>
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<table style="text-align: left;" border="1" cellpadding="2"
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cellspacing="2">
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<tr>
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<td style="vertical-align: top;">Start<br>
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</td>
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<td style="vertical-align: top;">94&nbsp; C<br>
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</td>
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<td style="vertical-align: top;">2 min<br>
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</td>
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</tr>
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<tr>
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<td style="vertical-align: top;">Denaturing<br>
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</td>
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<td style="vertical-align: top;">94 C<br>
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</td>
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<td style="vertical-align: top;">1 min<br>
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</td>
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</tr>
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<tr>
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<td style="vertical-align: top;">Annealing<br>
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</td>
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<td style="vertical-align: top;">55 C<br>
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</td>
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<td style="vertical-align: top;">1 min<br>
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</td>
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</tr>
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<tr>
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<td style="vertical-align: top;">Elongation<br>
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</td>
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<td style="vertical-align: top;">72 C<br>
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</td>
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<td style="vertical-align: top;">2:30 min<br>
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</td>
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</tr>
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<tr>
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<td style="vertical-align: top;">Goto2<br>
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</td>
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<td style="vertical-align: top;">rep<br>
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</td>
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<td style="vertical-align: top;">29x<br>
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</td>
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</tr>
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<tr>
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<td style="vertical-align: top;">End<br>
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</td>
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<td style="vertical-align: top;">72 C<br>
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</td>
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<td style="vertical-align: top;">3 min<br>
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</td>
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</tr>
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<tr>
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<td style="vertical-align: top;">Hold<br>
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</td>
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<td style="vertical-align: top;">4 C<br>
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</td>
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<td style="vertical-align: top;"><br>
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</td>
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</tr>
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</table>

Revision as of 15:36, 16 October 2010

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