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Team:MIT mmethods
From 2010.igem.org
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1.7 Collagen filling<br> | 1.7 Collagen filling<br> | ||
1.8 Cell Seeding<br> | 1.8 Cell Seeding<br> | ||
| + | 2 Protocol for Deflection Experiments<br> | ||
| + | 2.1 Tubing Setup Details<br> | ||
| + | 2.2 Adding Medium to Channels<br> | ||
| + | 2.3 Connecting device to pressure valve<br> | ||
| + | 2.4 Microcontroller details<br> | ||
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</div></td><tr><td> | </div></td><tr><td> | ||
Revision as of 01:55, 15 October 2010
iGEM 2010
Bacterial Construction Protocol
Bacterial Experimental Protocol
Phage western blot
Mammalian Protocol
Microfluidic stress
Materials
mammalian methods |
The Mammalian team focused on creating a pressure-sensitive promoter and creating a standard protocol for mammalian genes.
1 HTD Preparation Protocol 1.1 PDMS Mixture Preparation 1.2 PDMS Pouring 1.3 PDMS Baking 1.4 PDMS-Device punching and Bonding 1.5 PDMS Device Bonding 1.6 PDL coating 1.7 Collagen filling 1.8 Cell Seeding 2 Protocol for Deflection Experiments 2.1 Tubing Setup Details 2.2 Adding Medium to Channels 2.3 Connecting device to pressure valve 2.4 Microcontroller details |
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mammalian methods |
