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Team:MIT mmethods
From 2010.igem.org
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<div class="sidep" style="width:200px; display:inline; padding: 5px;"> | <div class="sidep" style="width:200px; display:inline; padding: 5px;"> | ||
1 HTD Preparation Protocol<br> | 1 HTD Preparation Protocol<br> | ||
| - | 1.1 PDMS Mixture Preparation<br> | + | 1.1 PDMS Mixture Preparation<br> |
| - | 1.2 PDMS Pouring<br> | + | 1.2 PDMS Pouring<br> |
| - | 1.3 PDMS Baking<br> | + | 1.3 PDMS Baking<br> |
| - | 1.4 PDMS-Device punching and Bonding<br> | + | 1.4 PDMS-Device punching and Bonding<br> |
| - | 1.5 PDMS Device Bonding<br> | + | 1.5 PDMS Device Bonding<br> |
| - | 1.6 PDL coating<br> | + | 1.6 PDL coating<br> |
| - | 1.7 Collagen filling<br> | + | 1.7 Collagen filling<br> |
| - | 1.8 Cell Seeding<br> | + | 1.8 Cell Seeding<br> |
</div> | </div> | ||
Revision as of 01:51, 15 October 2010
iGEM 2010
Bacterial Construction Protocol
Bacterial Experimental Protocol
Phage western blot
Mammalian Protocol
Microfluidic stress
Materials
mammalian methods |
The Mammalian team focused on creating a pressure-sensitive promoter and creating a standard protocol for mammalian genes.
1 HTD Preparation Protocol
1.1 PDMS Mixture Preparation 1.2 PDMS Pouring 1.3 PDMS Baking 1.4 PDMS-Device punching and Bonding 1.5 PDMS Device Bonding 1.6 PDL coating 1.7 Collagen filling 1.8 Cell Seeding mammalian methods |
