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Team:Caltech/Week 1
From 2010.igem.org
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*Made stock solutions for transformation and subsequent culturing | *Made stock solutions for transformation and subsequent culturing | ||
*Transformed XL-1 Blue E. Coli with selected BioBricks | *Transformed XL-1 Blue E. Coli with selected BioBricks | ||
| + | **J09250, K124017, K124014, R0082, R0015, R0034, pBAD, I13601 | ||
| + | |||
| + | |||
| + | ==Friday== | ||
| + | *Checked transformation | ||
| + | *Minipreped transformed bacteria | ||
| + | **Checked the plasmid concentration in the mini-prep solution | ||
| + | *Made glycerol stocks from the transformed plasmid | ||
Revision as of 23:40, 18 June 2010
Contents |
Monday
- Discussed procuring items & lab space.
- Prepared for SURF start.
Tuesday
- Created GANTT Chart (below) detailing our work plan for the summer.
- Detailed possible side projects, open to any member as time permits:
- Agarase
- pH/Environmental Response
- Phototaxis
- Colored plastic
- Activa (transglutaminase)
- Procured supplies from stock room
- Cleaned out lab space
- Prepared LB-amp media & plates
Wednesday
- Procured remaining antibiotics & sample of XL1 Blue cells
- Prepare orders for Sigma, NEB, and others
- Sigma: Crosslinking compounds, substrates (linseed oil & soybean oil)
- NEB: Biobrick assembly kit, Ladders, polymerase
- Prepared list of biobricks to order
- Addl Lysis bricks
- Agarase bricks
Thursday
- Made stock solutions for transformation and subsequent culturing
- Transformed XL-1 Blue E. Coli with selected BioBricks
- J09250, K124017, K124014, R0082, R0015, R0034, pBAD, I13601
Friday
- Checked transformation
- Minipreped transformed bacteria
- Checked the plasmid concentration in the mini-prep solution
- Made glycerol stocks from the transformed plasmid
