Team:TU Delft/18 June 2010 content
From 2010.igem.org
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We also booked a hotel in Boston! | We also booked a hotel in Boston! | ||
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+ | ===Lab work=== | ||
+ | Pieter and Eva worked on the emulsifying activity assay. Four samples were tested: | ||
+ | |||
+ | Sample #, Amount of SDS, Hexalkane | ||
+ | |||
+ | 1. Blank, 0 | ||
+ | |||
+ | 2. No SDS, 0.02 ml | ||
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+ | 3. 0,01% SDS, 0.02 ml | ||
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+ | 4. 1% SDS, 0.02 ml | ||
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+ | Tris buffer (pH 8) was added to a total volume of 7.5 ml. | ||
+ | |||
+ | Incubate 1h at 30C at shaken at 200 RPM. | ||
+ | |||
+ | Absorbance measured at 600 nm. | ||
+ | |||
+ | 1. 0 | ||
+ | |||
+ | 2. 0 | ||
+ | |||
+ | 3. 0.4 | ||
+ | |||
+ | 4. 0.2 |
Revision as of 14:36, 18 June 2010
We had a nice slow day, finishing some small experiments and reorganizing our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy.
We also booked a hotel in Boston!
Lab work
Pieter and Eva worked on the emulsifying activity assay. Four samples were tested:
Sample #, Amount of SDS, Hexalkane
1. Blank, 0
2. No SDS, 0.02 ml
3. 0,01% SDS, 0.02 ml
4. 1% SDS, 0.02 ml
Tris buffer (pH 8) was added to a total volume of 7.5 ml.
Incubate 1h at 30C at shaken at 200 RPM.
Absorbance measured at 600 nm.
1. 0
2. 0
3. 0.4
4. 0.2