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__NOTOC__{{UT-Tokyo_CSS3}}{{UT-Tokyo_Head}} <h1>Restriction Enzyme digestion —One by one</h1> <h2>Procedure（20μl）</h2> ! use 5ml tube<br /> *1. add　1ng　plasmid 　　　　　2μl　10xbuffer<br /> 　　　　　2.5μl　enzyme<br /> 　　　　　MilliQ up to 20μl<br /> *2. incubate at 37 degrees all night<br /> <br /> Plasmid・MilliQ<br /> EcoRI XbaI SpeI PstI Buffer H M(+0.01%BSA) M H <br /> buffer and activity of the restriction enzyme<br /> restriction enzyme relative activity（%）<br /> L M H K EcoRI (20) (100) 100 (120) SpeI (80) 100 80 100 PstI (<20) (60) 100 80 XbaI <20 80* 20 <20 * ＋0.01% BSA →100%
 Xba I<br /> ※（） tends to show the star activity<br /> {{UT-Tokyo_Foot}}

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