Team:UT-Tokyo/Restriction Enzyme digestion —One by one

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UT-Tokyo

Restriction Enzyme digestion —One by one

Procedure(20μl)

! use 5ml tube

  • 1. add 1ng plasmid

     2μl 10xbuffer
     2.5μl enzyme
     MilliQ up to 20μl

  • 2. incubate at 37 degrees all night


Plasmid・MilliQ
EcoRI XbaI SpeI PstI Buffer H M(+0.01%BSA) M H
buffer and activity of the restriction enzyme
restriction enzyme relative activity(%)
L M H K EcoRI (20) (100) 100 (120) SpeI (80) 100 80 100 PstI (<20) (60) 100 80 XbaI <20 80* 20 <20

  • +0.01% BSA →100%
 Xba I

※() tends to show the star activity