Team:INSA-Lyon/Protocols/Biofilms quantification

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<h3> Protocols </h3>
<br><p>Choose a protocol to read its description : 
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<h5 style="text-indent:20px">Biofilms quantification</h5>
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<li>Precultures of PHL818 and MG1655 in M63 medium + 0,2g/L glucose</li>
<li>Dilute the cultures in order to have an absorbance (600nm) of 0,02, into a 24-well hydrophobic polystyrene plate, in (M63 + glucose)/2 medium, with sucrose concentrations varying between 0 and 0,3mol/L</li>
<li>Incubate at 30°C, during 48h</li>
<li>Pull out the 2ml supernatants and rinse one time with 1ml of M63 medium.</li>
<li>Resuspend the adherent bacteria with 1ml of M63 medium.</li>
<li>Measure the biomass in 3ml supernatants and also in 1ml biofilm (absorbance at 600nm). Calculate the percentage of bacteria in biofilms, which represents the adherence percentage.</li>
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