"

From 2010.igem.org

	
		

	
	
		

		

			
  Home  
			
  Team  
					
			
			
			
  Project  
			
  Parts  
			
  Modeling  
			
  Notebook  
                        
  Safety  
                        
  Sponsors  

		
		
	

Quorum addiction module

• Verification by PCR of the ligations RBS1+LuxR, RBS0.3+ParD and RBS1+ParD. All of those failed.

Module hydrogen

• We continue the purification process for the deletion candidate
• We continue to try to obtain other deletion candidates
• We did not manage to ligate tdcE with the RBS. We started again the maxipreparation of the RBS

Module homologous recombination

• The cm resistance cassette was not inserted properly into the pSB1C3, we started again
• We digested gam and bet to insert them with a RBS

Module heavy metals detection

• We inserted the copper sensitive promoter in front of a RFP, we let the bacteria grow. Some of the bacteria turn red but the medium did not contain copper. We tested several candidates on a medium with copper, we let the bacteria grow

Other

• We try again to obtain more pSB1C3