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Quorum addiction module

• PCR to verify the ligations/electroporations of the 27/08.
• Re-ligations of the ones who failed.

Module hydrogen

• We continue the purification process for the deletion candidate
• We continue to try to obtain other deletion candidates
• We try again to ligate tdcE with the RBS

Module homologous recombination

• The cm resistance cassette was not inserted properly into the pSB1C3
• We obtained the gene gam and bet by PCR

Module heavy metals detection

• We inserted the gene of the carotene behind a constitutive promoter, we let the bacteria grow. The bacteria did not turn orange. We concluded that the gene of the carotene was a fail.
• We inserted the copper sensitive promoter in front of a RFP, we let the bacteria grow. Some of the bacteria turn red but the medium did not contain copper. We tested several candidates on a medium with copper

Other

• We try again to obtain more pSB1C3