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From 2010.igem.org

Knightmare:

Gel extraction and concentration determination of pduTUV PCR product in old standard.
The concentration is too low, repeat the PCR procedure.
PCR: pduTUV in old standard.

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gpw：

Overlap PCR：pduJK1,4 and pduJK5,6 following the old standards.

Colony PCR of pdu TUV after transfection,also following the old standards,negative results.