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From 2010.igem.org

Evelyn:

Restriction Enzyme Cutting of pduU-V and pduN-T.

Hilde:

select single colony from plate of transformation product of pduABJKNTUV

Colony PCR test for pduABJKNTUV

Transformation of product of ligation: pduABJKNTUV

Miniprep: plasmids of pduABJKNTUV