"

From 2010.igem.org

Hilde:

Transformation of the ligation product: pduABJKNTUV (Trans 10)

Coating of transformation product of pduABJKNTUV to Cl+ plate

Fastdigest cutting of pduNTUV (E,S)

DNA Ligation Kit LONG of pduABJK+pduNTUV (overnight)