"

From 2010.igem.org

Evelyn:

Genome extraction of C.freundii

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Knightmare:

PCR: pduN in old standard. Failed...
PCR: pduN in old standard. Temperature changed. Failed...
Suspectation: There's something wrong with TAE.

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LiuChang:

Continue the experiment of PCR of pduAB -- a set of experiments was designed to find out the reason of the prior failure in PCR.