"

From 2010.igem.org

Knightmare:

Bridging pduJK f1-r2 f3-r4 f5-r6 in old standard.
PCR: pduJK f1-r6 in old standard.
Digestion: pduJK with gel extraction and concentration determination.
Ligation: pduJK on PSB1C3.
Trasfermation: pduJK on PSB1C3.