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- Our project and design workplan has now been finalized!
- We are proud to present our main project, a genetic circuit which will enable a lawn of E. Coli to produce a spatial oscillating pattern (stripes) upon the initial stimulation of light to propagate the signaling cascade across the lawn.
- This project will build upon creating composite parts made from existing parts of the registry.
- Thus, we hydrated, transformed, cultured, and miniprepped the following parts: C0078, C0079, I12006, K274002, I14015, I13453, I15008, I15009, I15010, R0082, J23119, E1010
- Assembly workplan for light sensor is made:
- Assembling Part A: I13453 cut with Eco & Spe, RBS cut with Eco& Xba, I15009 cut with Xba & Pst, '1&2' cut with Spe & Pst
- Assembling Part B: RBS w/ Spe & Pst, I15009 w/ Xba & Pst
- Assembling Part C: RBS cut with Eco & Xba, J23119 cut w/ Eco & Spe
- ** Began assembling parts A&B
- Workplan for light receiver made: R0082-RBS-C0051-B0015
- Assembling RBS-C0051: RBS w/ Spe & Pst, C0051 w/ Xba & Pst
- Assembling RBS-C0051-B0015: RBS-C0051 w/ Eco & Spe, B0015 w/ Eco & Xba
- Assembling RBS-C0051-B0015 with R0082: RBS-C0051-B0015 w/ Xba & Pst, R0082 w/ Spe & Pst
- Assembling RBS-C0051-B0015 with I14015: RBS-C0051-B0015 w/ Xba & Pst, R0082 w/ Spe & Pst
- We must first make stocks of RBS-C0051 because it is used in multiple parts
RBS-C0051 will be used in creating the construct that will test/validate the initiator, the actual intiator, and the ON switch. Thus, there will be some slight collaboration in creating this part.
- Making: I12006-B0034-C0051-B0034-E10100-F1610
- Digested and purified: B0034, C0051, I12006
- Made competent cells of E. Coli Strain CP919
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We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!
We would also like to thank and acknowledge:
Our Advisors
Marc Facciotti
Ilias Tagkopoulos
Technical Guidance
David Larsen
Andrew Yao
Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China)
cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)
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