Team:Tsinghua/Notebook/11 August 2010
From 2010.igem.org
Module I, group 2(b)
Pick out 6 colonies to amplify. After 10 hours, purify the plasmids. Use PCR to identify P+E+M (E do & M do primers) and P+K+C (K up & C do primers) with two control (P+E & P+C with each pair of primers shown upon).
PCR system (SuperMix):
H2O 8.5μl primer1 0.5μl primer2 0.5μl template 0.5μl SuperMix 10μl Total 20μl
Run a gel. The order: P+E+M 1-6, P+E, Marker, P+K+C 1-6, P+C.
result
It seems that all the P+E+M are positive and the P+K+C are negative. We’ll ensure the result tomorrow.