Project I

Project II

Project I -- Lignin Terminator

Gene fragments are synthesized by GeneArt.

1 Shen-Long Tsai, Jeongseok Oh, Shailendra Singh, Ruizhen Chen, and Wilfred Chen, Functional Assembly of Minicellulosomes on the Saccharomyces cerevisiae Cell Surface for Cellulose Hydrolysis and Ethanol Production, Applied and Environmental Microbiology, October 2009, p. 6087-6093, Vol. 75, No. 19

2、Junji Ito, Akihiko Kosugi,2Tsutomu Tanaka, etc, Regulation of the Display Ratio of Enzymes on the Saccharomyces cerevisiae Cell Surface by the Immunoglobulin G and Cellulosomal Enzyme Binding Domains, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, June 2009, p. 4149–4154

3、Yasuya Fujita, Junji Ito, Mitsuyoshi Ueda, Hideki Fukuda,and Akihiko Kondo, Synergistic Saccharification, and Direct Fermentation to Ethanol, of Amorphous Cellulose by Use of an Engineered Yeast Strain Codisplaying Three Types of Cellulolytic Enzyme, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Feb. 2004, p. 1207–1212

4、Edward A Bayer , Raphael Lamed and Michael E Himmel, The potential of cellulases and cellulosomes for cellulosic waste management, Current Opinion in Biotechnology,Volume 18, Issue 3, June 2007, Pages 237-245

1. Construction of yeast surface display system plasmids containing four enzymes: Lip, MnP, Lac, Glox

2. Transforming to yeast

3. Detection of enzymes displayed using fluorescence antibody

4. Detection of enzymes' activity

5. Applying the system to practice, such as taking care of waste water from paper mills